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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella gallinarum Probable Fe (2+)-trafficking protein CSB-YP477586SWP
CSB-EP477586SWP
CSB-BP477586SWP
CSB-MP477586SWP
CSB-EP477586SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Bifunctional protein HldE (hldE) CSB-YP477587SWP
CSB-EP477587SWP
CSB-BP477587SWP
CSB-MP477587SWP
CSB-EP477587SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Putrescine aminotransferase (patA) CSB-YP477588SWP
CSB-EP477588SWP
CSB-BP477588SWP
CSB-MP477588SWP
CSB-EP477588SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum N-acetylmannosamine kinase (nanK) CSB-YP477589SWP
CSB-EP477589SWP
CSB-BP477589SWP
CSB-MP477589SWP
CSB-EP477589SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP477590SWP1
CSB-EP477590SWP1
CSB-BP477590SWP1
CSB-MP477590SWP1
CSB-EP477590SWP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Ribosomal protein L11 methyltransferase (prmA) CSB-YP477591SWP
CSB-EP477591SWP
CSB-BP477591SWP
CSB-MP477591SWP
CSB-EP477591SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum UPF0246 protein yaaA (yaaA) CSB-YP477592SWP
CSB-EP477592SWP
CSB-BP477592SWP
CSB-MP477592SWP
CSB-EP477592SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Isoleucine--tRNA ligase (ileS), partial CSB-YP477593SWP
CSB-EP477593SWP
CSB-BP477593SWP
CSB-MP477593SWP
CSB-EP477593SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L31 (rpmE) CSB-YP477594SWP
CSB-EP477594SWP
CSB-BP477594SWP
CSB-MP477594SWP
CSB-EP477594SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum L-rhamnose-proton symporter (rhaT), partial CSB-YP477595SWP
CSB-EP477595SWP
CSB-BP477595SWP
CSB-MP477595SWP
CSB-EP477595SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Rhamnulose-1-phosphate aldolase (rhaD) CSB-YP477596SWP
CSB-EP477596SWP
CSB-BP477596SWP
CSB-MP477596SWP
CSB-EP477596SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Endonuclease V (nfi) CSB-YP477597SWP
CSB-EP477597SWP
CSB-BP477597SWP
CSB-MP477597SWP
CSB-EP477597SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L7/L12 (rplL) CSB-YP477598SWP
CSB-EP477598SWP
CSB-BP477598SWP
CSB-MP477598SWP
CSB-EP477598SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum ATP-dependent RNA helicase rhlB (rhlB) CSB-YP477599SWP
CSB-EP477599SWP
CSB-BP477599SWP
CSB-MP477599SWP
CSB-EP477599SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP477600SWP
CSB-EP477600SWP
CSB-BP477600SWP
CSB-MP477600SWP
CSB-EP477600SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 50S ribosomal protein L34 (rpmH) CSB-YP477601SWP
CSB-EP477601SWP
CSB-BP477601SWP
CSB-MP477601SWP
CSB-EP477601SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Purine ribonucleoside efflux pump nepI (nepI), partial CSB-YP477602SWP
CSB-EP477602SWP
CSB-BP477602SWP
CSB-MP477602SWP
CSB-EP477602SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Dihydrodipicolinate reductase (dapB) CSB-YP477603SWP
CSB-EP477603SWP
CSB-BP477603SWP
CSB-MP477603SWP
CSB-EP477603SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Protein ApaG (apaG) CSB-YP477604SWP
CSB-EP477604SWP
CSB-BP477604SWP
CSB-MP477604SWP
CSB-EP477604SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Phosphopantetheine adenylyltransferase (coaD) CSB-YP477605SWP
CSB-EP477605SWP
CSB-BP477605SWP
CSB-MP477605SWP
CSB-EP477605SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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