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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella heidelberg 30S ribosomal protein S14 (rpsN) CSB-YP476906SWQ
CSB-EP476906SWQ
CSB-BP476906SWQ
CSB-MP476906SWQ
CSB-EP476906SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Sulfurtransferase TusD (tusD) CSB-YP476907SWQ
CSB-EP476907SWQ
CSB-BP476907SWQ
CSB-MP476907SWQ
CSB-EP476907SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0270 protein YheU (yheU) CSB-YP476908SWQ
CSB-EP476908SWQ
CSB-BP476908SWQ
CSB-MP476908SWQ
CSB-EP476908SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Phosphoenolpyruvate carboxykinase [ATP] (pckA), partial CSB-YP476909SWQ
CSB-EP476909SWQ
CSB-BP476909SWQ
CSB-MP476909SWQ
CSB-EP476909SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP476910SWV
CSB-EP476910SWV
CSB-BP476910SWV
CSB-MP476910SWV
CSB-EP476910SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP476911SWV
CSB-EP476911SWV
CSB-BP476911SWV
CSB-MP476911SWV
CSB-EP476911SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP476912SWV
CSB-EP476912SWV
CSB-BP476912SWV
CSB-MP476912SWV
CSB-EP476912SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L31 type B (rpmE2) CSB-YP476913SWV
CSB-EP476913SWV
CSB-BP476913SWV
CSB-MP476913SWV
CSB-EP476913SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund E3 ubiquitin-protein ligase SopA (sopA), partial CSB-YP476914SWV
CSB-EP476914SWV
CSB-BP476914SWV
CSB-MP476914SWV
CSB-EP476914SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Chaperone protein TorD (torD) CSB-YP476915SWV
CSB-EP476915SWV
CSB-BP476915SWV
CSB-MP476915SWV
CSB-EP476915SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund ATP synthase gamma chain (atpG) CSB-YP476916SWV
CSB-EP476916SWV
CSB-BP476916SWV
CSB-MP476916SWV
CSB-EP476916SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP476917SWV
CSB-EP476917SWV
CSB-BP476917SWV
CSB-MP476917SWV
CSB-EP476917SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP476918SWV
CSB-EP476918SWV
CSB-BP476918SWV
CSB-MP476918SWV
CSB-EP476918SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Probable endonuclease 4 (nfo) CSB-YP476919SWV
CSB-EP476919SWV
CSB-BP476919SWV
CSB-MP476919SWV
CSB-EP476919SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Proofreading thioesterase EntH (entH) CSB-YP476920SWV
CSB-EP476920SWV
CSB-BP476920SWV
CSB-MP476920SWV
CSB-EP476920SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP476921SWV
CSB-EP476921SWV
CSB-BP476921SWV
CSB-MP476921SWV
CSB-EP476921SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP476922SWV
CSB-EP476922SWV
CSB-BP476922SWV
CSB-MP476922SWV
CSB-EP476922SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP476923SWV
CSB-EP476923SWV
CSB-BP476923SWV
CSB-MP476923SWV
CSB-EP476923SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Protein FdhE (fdhE) CSB-YP476924SWV
CSB-EP476924SWV
CSB-BP476924SWV
CSB-MP476924SWV
CSB-EP476924SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L31 (rpmE) CSB-YP476925SWV
CSB-EP476925SWV
CSB-BP476925SWV
CSB-MP476925SWV
CSB-EP476925SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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