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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella schwarzengrund 50S ribosomal protein L9 (rplI) CSB-YP476946SWV
CSB-EP476946SWV
CSB-BP476946SWV
CSB-MP476946SWV
CSB-EP476946SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Aspartate carbamoyltransferase (pyrB) CSB-YP476947SWV
CSB-EP476947SWV
CSB-BP476947SWV
CSB-MP476947SWV
CSB-EP476947SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund tRNA pseudouridine synthase D (truD) CSB-YP476948SWV
CSB-EP476948SWV
CSB-BP476948SWV
CSB-MP476948SWV
CSB-EP476948SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP476949SWV
CSB-EP476949SWV
CSB-BP476949SWV
CSB-MP476949SWV
CSB-EP476949SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Non-canonical purine NTP phosphatase (yjjX) CSB-YP476950SWV
CSB-EP476950SWV
CSB-BP476950SWV
CSB-MP476950SWV
CSB-EP476950SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund NH (3)-dependent NAD (+) synthetase CSB-YP476951SWV
CSB-EP476951SWV
CSB-BP476951SWV
CSB-MP476951SWV
CSB-EP476951SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP476952SWV
CSB-EP476952SWV
CSB-BP476952SWV
CSB-MP476952SWV
CSB-EP476952SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund DNA mismatch repair protein mutH (mutH) CSB-YP476953SWV
CSB-EP476953SWV
CSB-BP476953SWV
CSB-MP476953SWV
CSB-EP476953SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 3-dehydroquinate dehydratase (aroD) CSB-YP476954SWV
CSB-EP476954SWV
CSB-BP476954SWV
CSB-MP476954SWV
CSB-EP476954SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Electron transport complex protein RnfD (rnfD), partial CSB-YP476955SWV
CSB-EP476955SWV
CSB-BP476955SWV
CSB-MP476955SWV
CSB-EP476955SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP476956SWV
CSB-EP476956SWV
CSB-BP476956SWV
CSB-MP476956SWV
CSB-EP476956SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Protein sprT (sprT) CSB-YP476957SWV
CSB-EP476957SWV
CSB-BP476957SWV
CSB-MP476957SWV
CSB-EP476957SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0482 protein ynfB (ynfB) CSB-YP476958SWV
CSB-EP476958SWV
CSB-BP476958SWV
CSB-MP476958SWV
CSB-EP476958SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Undecaprenyl-diphosphatase (uppP), partial CSB-YP476959SWV1
CSB-EP476959SWV1
CSB-BP476959SWV1
CSB-MP476959SWV1
CSB-EP476959SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0246 protein yaaA (yaaA) CSB-YP476960SWV
CSB-EP476960SWV
CSB-BP476960SWV
CSB-MP476960SWV
CSB-EP476960SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund D-tagatose-1,6-bisphosphate aldolase subunit gatZ (gatZ) CSB-YP476961SWV
CSB-EP476961SWV
CSB-BP476961SWV
CSB-MP476961SWV
CSB-EP476961SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP476962SWV
CSB-EP476962SWV
CSB-BP476962SWV
CSB-MP476962SWV
CSB-EP476962SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Transcriptional regulator NanR (nanR) CSB-YP476963SWV
CSB-EP476963SWV
CSB-BP476963SWV
CSB-MP476963SWV
CSB-EP476963SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 30S ribosomal protein S20 (rpsT) CSB-YP476964SWV
CSB-EP476964SWV
CSB-BP476964SWV
CSB-MP476964SWV
CSB-EP476964SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund L-carnitine/gamma-butyrobetaine antiporter (caiT), partial CSB-YP476965SWV
CSB-EP476965SWV
CSB-BP476965SWV
CSB-MP476965SWV
CSB-EP476965SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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