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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Anoxybacillus flavithermus Aspartate 1-decarboxylase (panD) CSB-YP478466BZO
CSB-EP478466BZO
CSB-BP478466BZO
CSB-MP478466BZO
CSB-EP478466BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus UPF0291 protein Aflv_1503 (Aflv_1503) CSB-YP478467BZO
CSB-EP478467BZO
CSB-BP478467BZO
CSB-MP478467BZO
CSB-EP478467BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus N-acetyldiaminopimelate deacetylase (Aflv_1903) CSB-YP478468BZO
CSB-EP478468BZO
CSB-BP478468BZO
CSB-MP478468BZO
CSB-EP478468BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 50S ribosomal protein L27 (rpmA) CSB-YP478469BZO
CSB-EP478469BZO
CSB-BP478469BZO
CSB-MP478469BZO
CSB-EP478469BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Uridine kinase (udk) CSB-YP478470BZO
CSB-EP478470BZO
CSB-BP478470BZO
CSB-MP478470BZO
CSB-EP478470BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP478471BZO
CSB-EP478471BZO
CSB-BP478471BZO
CSB-MP478471BZO
CSB-EP478471BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 50S ribosomal protein L7/L12 (rplL) CSB-YP478472BZO
CSB-EP478472BZO
CSB-BP478472BZO
CSB-MP478472BZO
CSB-EP478472BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S3 (rpsC) CSB-YP478473BZO
CSB-EP478473BZO
CSB-BP478473BZO
CSB-MP478473BZO
CSB-EP478473BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 50S ribosomal protein L24 (rplX) CSB-YP478474BZO
CSB-EP478474BZO
CSB-BP478474BZO
CSB-MP478474BZO
CSB-EP478474BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S8 (rpsH) CSB-YP478475BZO
CSB-EP478475BZO
CSB-BP478475BZO
CSB-MP478475BZO
CSB-EP478475BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S13 (rpsM) CSB-YP478476BZO
CSB-EP478476BZO
CSB-BP478476BZO
CSB-MP478476BZO
CSB-EP478476BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP478477BZO
CSB-EP478477BZO
CSB-BP478477BZO
CSB-MP478477BZO
CSB-EP478477BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Putative membrane protein insertion efficiency factor (Aflv_0398) CSB-YP478478BZO
CSB-EP478478BZO
CSB-BP478478BZO
CSB-MP478478BZO
CSB-EP478478BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP478479BZO
CSB-EP478479BZO
CSB-BP478479BZO
CSB-MP478479BZO
CSB-EP478479BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Anti-sigma F factor (spoIIAB) CSB-YP478480BZO
CSB-EP478480BZO
CSB-BP478480BZO
CSB-MP478480BZO
CSB-EP478480BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Small, acid-soluble spore protein N (sspN) CSB-YP478481BZO
CSB-EP478481BZO
CSB-BP478481BZO
CSB-MP478481BZO
CSB-EP478481BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus ATP phosphoribosyltransferase (hisG) CSB-YP478482BZO
CSB-EP478482BZO
CSB-BP478482BZO
CSB-MP478482BZO
CSB-EP478482BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Lactate utilization protein A (lutA) CSB-YP478483BZO
CSB-EP478483BZO
CSB-BP478483BZO
CSB-MP478483BZO
CSB-EP478483BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Cobalt transport protein CbiM (cbiM), partial CSB-YP478484BZO1
CSB-EP478484BZO1
CSB-BP478484BZO1
CSB-MP478484BZO1
CSB-EP478484BZO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP478485BZO
CSB-EP478485BZO
CSB-BP478485BZO
CSB-MP478485BZO
CSB-EP478485BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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