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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rhizobium leguminosarum bv. trifolii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP478006RKQ
CSB-EP478006RKQ
CSB-BP478006RKQ
CSB-MP478006RKQ
CSB-EP478006RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Cobalamin synthase (cobS), partial CSB-YP478007RKQ1
CSB-EP478007RKQ1
CSB-BP478007RKQ1
CSB-MP478007RKQ1
CSB-EP478007RKQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP478008RKQ
CSB-EP478008RKQ
CSB-BP478008RKQ
CSB-MP478008RKQ
CSB-EP478008RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 30S ribosomal protein S16 (rpsP) CSB-YP478009RKQ
CSB-EP478009RKQ
CSB-BP478009RKQ
CSB-MP478009RKQ
CSB-EP478009RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Homoserine O-succinyltransferase (metA) CSB-YP478010RKQ
CSB-EP478010RKQ
CSB-BP478010RKQ
CSB-MP478010RKQ
CSB-EP478010RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Glutamate 5-kinase (proB) CSB-YP478011RKQ
CSB-EP478011RKQ
CSB-BP478011RKQ
CSB-MP478011RKQ
CSB-EP478011RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Betaine aldehyde dehydrogenase (betB) CSB-YP478012RKQ
CSB-EP478012RKQ
CSB-BP478012RKQ
CSB-MP478012RKQ
CSB-EP478012RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Leucine--tRNA ligase (leuS), partial CSB-YP478013RKQ
CSB-EP478013RKQ
CSB-BP478013RKQ
CSB-MP478013RKQ
CSB-EP478013RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP478014RKQ
CSB-EP478014RKQ
CSB-BP478014RKQ
CSB-MP478014RKQ
CSB-EP478014RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP478015RKQ
CSB-EP478015RKQ
CSB-BP478015RKQ
CSB-MP478015RKQ
CSB-EP478015RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP478016RKQ
CSB-EP478016RKQ
CSB-BP478016RKQ
CSB-MP478016RKQ
CSB-EP478016RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Ribosome-binding factor A (rbfA) CSB-YP478017RKQ
CSB-EP478017RKQ
CSB-BP478017RKQ
CSB-MP478017RKQ
CSB-EP478017RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP478018RKQ
CSB-EP478018RKQ
CSB-BP478018RKQ
CSB-MP478018RKQ
CSB-EP478018RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii DNA replication and repair protein recF (recF) CSB-YP478019RKQ
CSB-EP478019RKQ
CSB-BP478019RKQ
CSB-MP478019RKQ
CSB-EP478019RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Transcription elongation factor GreA (greA) CSB-YP478020RKQ
CSB-EP478020RKQ
CSB-BP478020RKQ
CSB-MP478020RKQ
CSB-EP478020RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP478021RKQ
CSB-EP478021RKQ
CSB-BP478021RKQ
CSB-MP478021RKQ
CSB-EP478021RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP478022RKQ
CSB-EP478022RKQ
CSB-BP478022RKQ
CSB-MP478022RKQ
CSB-EP478022RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP478023RKQ
CSB-EP478023RKQ
CSB-BP478023RKQ
CSB-MP478023RKQ
CSB-EP478023RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Aspartate carbamoyltransferase (pyrB) CSB-YP478024RKQ
CSB-EP478024RKQ
CSB-BP478024RKQ
CSB-MP478024RKQ
CSB-EP478024RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 30S ribosomal protein S7 (rpsG) CSB-YP478025RKQ
CSB-EP478025RKQ
CSB-BP478025RKQ
CSB-MP478025RKQ
CSB-EP478025RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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