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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Glucans biosynthesis protein C (mdoC), partial CSB-YP478206ENW
CSB-EP478206ENW
CSB-BP478206ENW
CSB-MP478206ENW
CSB-EP478206ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L32 (rpmF) CSB-YP478207ENW
CSB-EP478207ENW
CSB-BP478207ENW
CSB-MP478207ENW
CSB-EP478207ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli High frequency lysogenization protein HflD (hflD) CSB-YP478208ENW
CSB-EP478208ENW
CSB-BP478208ENW
CSB-MP478208ENW
CSB-EP478208ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fatty acid metabolism regulator protein (fadR) CSB-YP478209ENW
CSB-EP478209ENW
CSB-BP478209ENW
CSB-MP478209ENW
CSB-EP478209ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamyl-tRNA reductase (hemA) CSB-YP478210ENW
CSB-EP478210ENW
CSB-BP478210ENW
CSB-MP478210ENW
CSB-EP478210ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0263 protein yciU (yciU) CSB-YP478211ENW
CSB-EP478211ENW
CSB-BP478211ENW
CSB-MP478211ENW
CSB-EP478211ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli GTP cyclohydrolase-2 (ribA) CSB-YP478212ENW
CSB-EP478212ENW
CSB-BP478212ENW
CSB-MP478212ENW
CSB-EP478212ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP478213ENW
CSB-EP478213ENW
CSB-BP478213ENW
CSB-MP478213ENW
CSB-EP478213ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0060 membrane protein ynfA (ynfA), partial CSB-YP478214ENW1
CSB-EP478214ENW1
CSB-BP478214ENW1
CSB-MP478214ENW1
CSB-EP478214ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP478215ENW
CSB-EP478215ENW
CSB-BP478215ENW
CSB-MP478215ENW
CSB-EP478215ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-dehydroquinate dehydratase (aroD) CSB-YP478216ENW
CSB-EP478216ENW
CSB-BP478216ENW
CSB-MP478216ENW
CSB-EP478216ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein ves (ves) CSB-YP478217ENW
CSB-EP478217ENW
CSB-BP478217ENW
CSB-MP478217ENW
CSB-EP478217ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0229 protein yeaH (yeaH) CSB-YP478218ENW
CSB-EP478218ENW
CSB-BP478218ENW
CSB-MP478218ENW
CSB-EP478218ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protease HtpX (htpX), partial CSB-YP478219ENW1
CSB-EP478219ENW1
CSB-BP478219ENW1
CSB-MP478219ENW1
CSB-EP478219ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP478220RLY
CSB-EP478220RLY
CSB-BP478220RLY
CSB-MP478220RLY
CSB-EP478220RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Putative Holliday junction resolvase (RC1_1518) CSB-YP478221RLY
CSB-EP478221RLY
CSB-BP478221RLY
CSB-MP478221RLY
CSB-EP478221RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP478222RLY
CSB-EP478222RLY
CSB-BP478222RLY
CSB-MP478222RLY
CSB-EP478222RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Thiamine-phosphate synthase (thiE) CSB-YP478223RLY
CSB-EP478223RLY
CSB-BP478223RLY
CSB-MP478223RLY
CSB-EP478223RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Diaminopimelate epimerase (dapF) CSB-YP478224RLY
CSB-EP478224RLY
CSB-BP478224RLY
CSB-MP478224RLY
CSB-EP478224RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum ATP synthase gamma chain (atpG) CSB-YP478225RLY
CSB-EP478225RLY
CSB-BP478225RLY
CSB-MP478225RLY
CSB-EP478225RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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