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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP478406DPP
CSB-EP478406DPP
CSB-BP478406DPP
CSB-MP478406DPP
CSB-EP478406DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase alpha chain (trpA) CSB-YP478407DPP
CSB-EP478407DPP
CSB-BP478407DPP
CSB-MP478407DPP
CSB-EP478407DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP478408DPP
CSB-EP478408DPP
CSB-BP478408DPP
CSB-MP478408DPP
CSB-EP478408DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase (pyrB) CSB-YP478409DPP
CSB-EP478409DPP
CSB-BP478409DPP
CSB-MP478409DPP
CSB-EP478409DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein (nifH) CSB-YP478410DPP
CSB-EP478410DPP
CSB-BP478410DPP
CSB-MP478410DPP
CSB-EP478410DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP478411DPP
CSB-EP478411DPP
CSB-BP478411DPP
CSB-MP478411DPP
CSB-EP478411DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (ligA), partial CSB-YP478412DPP
CSB-EP478412DPP
CSB-BP478412DPP
CSB-MP478412DPP
CSB-EP478412DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP478413DPP
CSB-EP478413DPP
CSB-BP478413DPP
CSB-MP478413DPP
CSB-EP478413DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pantothenate synthetase (panC) CSB-YP478414DPP
CSB-EP478414DPP
CSB-BP478414DPP
CSB-MP478414DPP
CSB-EP478414DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L10e (rpl10e) CSB-YP478415TMR
CSB-EP478415TMR
CSB-BP478415TMR
CSB-MP478415TMR
CSB-EP478415TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S3P (rps3p) CSB-YP478416TMR
CSB-EP478416TMR
CSB-BP478416TMR
CSB-MP478416TMR
CSB-EP478416TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L32e (rpl32e) CSB-YP478417TMR
CSB-EP478417TMR
CSB-BP478417TMR
CSB-MP478417TMR
CSB-EP478417TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S4P (rps4p) CSB-YP478418TMR
CSB-EP478418TMR
CSB-BP478418TMR
CSB-MP478418TMR
CSB-EP478418TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Signal recognition 54 kDa protein (srp54) CSB-YP478419TMR
CSB-EP478419TMR
CSB-BP478419TMR
CSB-MP478419TMR
CSB-EP478419TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L15e (rpl15e) CSB-YP478420TMR
CSB-EP478420TMR
CSB-BP478420TMR
CSB-MP478420TMR
CSB-EP478420TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L11P (rpl11p) CSB-YP478421TMR
CSB-EP478421TMR
CSB-BP478421TMR
CSB-MP478421TMR
CSB-EP478421TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable thymidylate kinase (tmk) CSB-YP478422TMR
CSB-EP478422TMR
CSB-BP478422TMR
CSB-MP478422TMR
CSB-EP478422TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 30S ribosomal protein S7P (rps7p) CSB-YP478423TMR
CSB-EP478423TMR
CSB-BP478423TMR
CSB-MP478423TMR
CSB-EP478423TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP478424TMR
CSB-EP478424TMR
CSB-BP478424TMR
CSB-MP478424TMR
CSB-EP478424TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable L-threonine 3-dehydrogenase (tdh) CSB-YP478425TMR
CSB-EP478425TMR
CSB-BP478425TMR
CSB-MP478425TMR
CSB-EP478425TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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