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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aliivibrio salmonicida 50S ribosomal protein L28 (rpmB) CSB-YP478106AZM
CSB-EP478106AZM
CSB-BP478106AZM
CSB-MP478106AZM
CSB-EP478106AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 30S ribosomal protein S10 (rpsJ) CSB-YP478107AZM
CSB-EP478107AZM
CSB-BP478107AZM
CSB-MP478107AZM
CSB-EP478107AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L22 (rplV) CSB-YP478108AZM
CSB-EP478108AZM
CSB-BP478108AZM
CSB-MP478108AZM
CSB-EP478108AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L6 (rplF) CSB-YP478109AZM
CSB-EP478109AZM
CSB-BP478109AZM
CSB-MP478109AZM
CSB-EP478109AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Probable transcriptional regulatory protein VSAL_II0141 (VSAL_II0141) CSB-YP478110AZM
CSB-EP478110AZM
CSB-BP478110AZM
CSB-MP478110AZM
CSB-EP478110AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Dihydroorotase (pyrC) CSB-YP478111AZM
CSB-EP478111AZM
CSB-BP478111AZM
CSB-MP478111AZM
CSB-EP478111AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0265 protein VSAL_II0603 (VSAL_II0603) CSB-YP478112AZM
CSB-EP478112AZM
CSB-BP478112AZM
CSB-MP478112AZM
CSB-EP478112AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Chromosome partition protein mukE (mukE) CSB-YP478113AZM
CSB-EP478113AZM
CSB-BP478113AZM
CSB-MP478113AZM
CSB-EP478113AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Adenylosuccinate synthetase (Pc06g01770) CSB-YP478114ETH
CSB-EP478114ETH
CSB-BP478114ETH
CSB-MP478114ETH
CSB-EP478114ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Putative metallocarboxypeptidase ecm14 (ecm14), partial CSB-YP478115ETH
CSB-EP478115ETH
CSB-BP478115ETH
CSB-MP478115ETH
CSB-EP478115ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Nucleolar protein 9 (nop9), partial CSB-YP478116ETH
CSB-EP478116ETH
CSB-BP478116ETH
CSB-MP478116ETH
CSB-EP478116ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Glutamyl-tRNA (Gln) amidotransferase subunit B, mitochondrial CSB-YP478117ETH
CSB-EP478117ETH
CSB-BP478117ETH
CSB-MP478117ETH
CSB-EP478117ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Probable cytosolic iron-sulfur protein assembly protein 1 (cia1) CSB-YP478118ETH
CSB-EP478118ETH
CSB-BP478118ETH
CSB-MP478118ETH
CSB-EP478118ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Putative glutathione-dependent formaldehyde-activating enzyme (Pc20g15670) CSB-YP478119ETH
CSB-EP478119ETH
CSB-BP478119ETH
CSB-MP478119ETH
CSB-EP478119ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Cyanate hydratase (cyn1) CSB-YP478120ETH
CSB-EP478120ETH
CSB-BP478120ETH
CSB-MP478120ETH
CSB-EP478120ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Methylthioribulose-1-phosphate dehydratase (mde1) CSB-YP478121ETH
CSB-EP478121ETH
CSB-BP478121ETH
CSB-MP478121ETH
CSB-EP478121ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Probable Xaa-Pro aminopeptidase P (ampp), partial CSB-YP478122ETH
CSB-EP478122ETH
CSB-BP478122ETH
CSB-MP478122ETH
CSB-EP478122ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Protein get1 (get1), partial CSB-YP478123ETH1
CSB-EP478123ETH1
CSB-BP478123ETH1
CSB-MP478123ETH1
CSB-EP478123ETH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP478124ENW
CSB-EP478124ENW
CSB-BP478124ENW
CSB-MP478124ENW
CSB-EP478124ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP478125ENW
CSB-EP478125ENW
CSB-BP478125ENW
CSB-MP478125ENW
CSB-EP478125ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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