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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Protein viaA (viaA) CSB-YP478166ENW
CSB-EP478166ENW
CSB-BP478166ENW
CSB-MP478166ENW
CSB-EP478166ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP478167ENW
CSB-EP478167ENW
CSB-BP478167ENW
CSB-MP478167ENW
CSB-EP478167ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putrescine aminotransferase (patA) CSB-YP478168ENW
CSB-EP478168ENW
CSB-BP478168ENW
CSB-MP478168ENW
CSB-EP478168ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP478169ENW
CSB-EP478169ENW
CSB-BP478169ENW
CSB-MP478169ENW
CSB-EP478169ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Tyrosine recombinase XerC (xerC) CSB-YP478170ENW
CSB-EP478170ENW
CSB-BP478170ENW
CSB-MP478170ENW
CSB-EP478170ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fatty acid oxidation complex subunit alpha (fadB) CSB-YP478171ENW
CSB-EP478171ENW
CSB-BP478171ENW
CSB-MP478171ENW
CSB-EP478171ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein FdhE (fdhE) CSB-YP478172ENW
CSB-EP478172ENW
CSB-BP478172ENW
CSB-MP478172ENW
CSB-EP478172ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli CDP-diacylglycerol pyrophosphatase (cdh), partial CSB-YP478173ENW
CSB-EP478173ENW
CSB-BP478173ENW
CSB-MP478173ENW
CSB-EP478173ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Penicillin-insensitive murein endopeptidase (mepA) CSB-YP478174ENW
CSB-EP478174ENW
CSB-BP478174ENW
CSB-MP478174ENW
CSB-EP478174ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein CysZ (cysZ), partial CSB-YP478175ENW1
CSB-EP478175ENW1
CSB-BP478175ENW1
CSB-MP478175ENW1
CSB-EP478175ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0370 protein ypfN (ypfN), partial CSB-YP478176ENW
CSB-EP478176ENW
CSB-BP478176ENW
CSB-MP478176ENW
CSB-EP478176ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP478177ENW
CSB-EP478177ENW
CSB-BP478177ENW
CSB-MP478177ENW
CSB-EP478177ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Histidine--tRNA ligase (hisS) CSB-YP478178ENW
CSB-EP478178ENW
CSB-BP478178ENW
CSB-MP478178ENW
CSB-EP478178ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Chaperone protein hscA (hscA) CSB-YP478179ENW
CSB-EP478179ENW
CSB-BP478179ENW
CSB-MP478179ENW
CSB-EP478179ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase (hcaB) CSB-YP478180ENW
CSB-EP478180ENW
CSB-BP478180ENW
CSB-MP478180ENW
CSB-EP478180ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamate racemase (murI) CSB-YP478181ENW
CSB-EP478181ENW
CSB-BP478181ENW
CSB-MP478181ENW
CSB-EP478181ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP478182ENW
CSB-EP478182ENW
CSB-BP478182ENW
CSB-MP478182ENW
CSB-EP478182ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP478183ENW1
CSB-EP478183ENW1
CSB-BP478183ENW1
CSB-MP478183ENW1
CSB-EP478183ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S16 (rpsP) CSB-YP478184ENW
CSB-EP478184ENW
CSB-BP478184ENW
CSB-MP478184ENW
CSB-EP478184ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein nrdI (nrdI) CSB-YP478185ENW
CSB-EP478185ENW
CSB-BP478185ENW
CSB-MP478185ENW
CSB-EP478185ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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