Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Dihydrodipicolinate reductase (dapB) CSB-YP478126ENW
CSB-EP478126ENW
CSB-BP478126ENW
CSB-MP478126ENW
CSB-EP478126ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutathione-regulated potassium-efflux system ancillary protein kefF (kefF) CSB-YP478127ENW
CSB-EP478127ENW
CSB-BP478127ENW
CSB-MP478127ENW
CSB-EP478127ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Isoleucine--tRNA ligase (ileS), partial CSB-YP478128ENW
CSB-EP478128ENW
CSB-BP478128ENW
CSB-MP478128ENW
CSB-EP478128ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribulokinase (araB) CSB-YP478129ENW
CSB-EP478129ENW
CSB-BP478129ENW
CSB-MP478129ENW
CSB-EP478129ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP478130ENW
CSB-EP478130ENW
CSB-BP478130ENW
CSB-MP478130ENW
CSB-EP478130ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli GMP reductase (guaC) CSB-YP478131ENW
CSB-EP478131ENW
CSB-BP478131ENW
CSB-MP478131ENW
CSB-EP478131ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP478132ENW
CSB-EP478132ENW
CSB-BP478132ENW
CSB-MP478132ENW
CSB-EP478132ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli [Protein-PII] uridylyltransferase (glnD), partial CSB-YP478133ENW
CSB-EP478133ENW
CSB-BP478133ENW
CSB-MP478133ENW
CSB-EP478133ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP478134ENW
CSB-EP478134ENW
CSB-BP478134ENW
CSB-MP478134ENW
CSB-EP478134ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Shikimate kinase 2 (aroL) CSB-YP478135ENW
CSB-EP478135ENW
CSB-BP478135ENW
CSB-MP478135ENW
CSB-EP478135ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP478136ENW
CSB-EP478136ENW
CSB-BP478136ENW
CSB-MP478136ENW
CSB-EP478136ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Trigger factor (tig) CSB-YP478137ENW
CSB-EP478137ENW
CSB-BP478137ENW
CSB-MP478137ENW
CSB-EP478137ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cyanate hydratase (cynS) CSB-YP478138ENW
CSB-EP478138ENW
CSB-BP478138ENW
CSB-MP478138ENW
CSB-EP478138ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Esterase FrsA (frsA) CSB-YP478139ENW
CSB-EP478139ENW
CSB-BP478139ENW
CSB-MP478139ENW
CSB-EP478139ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable HTH-type transcriptional regulator matA homolog (matA) CSB-YP478140ENW
CSB-EP478140ENW
CSB-BP478140ENW
CSB-MP478140ENW
CSB-EP478140ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0133 protein ybaB (ybaB) CSB-YP478141ENW
CSB-EP478141ENW
CSB-BP478141ENW
CSB-MP478141ENW
CSB-EP478141ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Allantoinase (allB) CSB-YP478142ENW
CSB-EP478142ENW
CSB-BP478142ENW
CSB-MP478142ENW
CSB-EP478142ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Arginine--tRNA ligase (argS) CSB-YP478143ENW
CSB-EP478143ENW
CSB-BP478143ENW
CSB-MP478143ENW
CSB-EP478143ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein CrcB homolog (crcB), partial CSB-YP478144ENW1
CSB-EP478144ENW1
CSB-BP478144ENW1
CSB-MP478144ENW1
CSB-EP478144ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli LPS-assembly lipoprotein lptE (lptE) CSB-YP478145ENW
CSB-EP478145ENW
CSB-BP478145ENW
CSB-MP478145ENW
CSB-EP478145ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>