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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermococcus onnurineus DNA-directed RNA polymerase subunit P (rpoP) CSB-YP478426TMR
CSB-EP478426TMR
CSB-BP478426TMR
CSB-MP478426TMR
CSB-EP478426TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Enolase (eno) CSB-YP478427TMR
CSB-EP478427TMR
CSB-BP478427TMR
CSB-MP478427TMR
CSB-EP478427TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Tryptophan--tRNA ligase (trpS) CSB-YP478428TMR
CSB-EP478428TMR
CSB-BP478428TMR
CSB-MP478428TMR
CSB-EP478428TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus UPF0127 protein TON_0581 (TON_0581) CSB-YP478429TMR
CSB-EP478429TMR
CSB-BP478429TMR
CSB-MP478429TMR
CSB-EP478429TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Putative snRNP Sm-like protein (TON_0644) CSB-YP478430TMR
CSB-EP478430TMR
CSB-BP478430TMR
CSB-MP478430TMR
CSB-EP478430TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus V-type ATP synthase subunit C (atpC) CSB-YP478431TMR
CSB-EP478431TMR
CSB-BP478431TMR
CSB-MP478431TMR
CSB-EP478431TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Ribonuclease P protein component 2 (rnp2) CSB-YP478432TMR
CSB-EP478432TMR
CSB-BP478432TMR
CSB-MP478432TMR
CSB-EP478432TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Cyclic 2,3-diphosphoglycerate synthetase (cpgS) CSB-YP478433TMR
CSB-EP478433TMR
CSB-BP478433TMR
CSB-MP478433TMR
CSB-EP478433TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP478434TMR
CSB-EP478434TMR
CSB-BP478434TMR
CSB-MP478434TMR
CSB-EP478434TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus tRNA (pseudouridine (54)-N (1))-methyltransferase CSB-YP478435TMR
CSB-EP478435TMR
CSB-BP478435TMR
CSB-MP478435TMR
CSB-EP478435TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Translation initiation factor 2 subunit gamma (eIF2G) CSB-YP478436TMR
CSB-EP478436TMR
CSB-BP478436TMR
CSB-MP478436TMR
CSB-EP478436TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP478437TMR
CSB-EP478437TMR
CSB-BP478437TMR
CSB-MP478437TMR
CSB-EP478437TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Glyoxylate reductase (gyaR) CSB-YP478438TMR
CSB-EP478438TMR
CSB-BP478438TMR
CSB-MP478438TMR
CSB-EP478438TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable tyrosine recombinase XerC-like (TON_1013) CSB-YP478439TMR
CSB-EP478439TMR
CSB-BP478439TMR
CSB-MP478439TMR
CSB-EP478439TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus DNA-binding protein TON_1102 (TON_1102) CSB-YP478440TMR
CSB-EP478440TMR
CSB-BP478440TMR
CSB-MP478440TMR
CSB-EP478440TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 3-dehydroquinate dehydratase (aroD) CSB-YP478441TMR
CSB-EP478441TMR
CSB-BP478441TMR
CSB-MP478441TMR
CSB-EP478441TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable RNA 2'-phosphotransferase (kptA) CSB-YP478442TMR
CSB-EP478442TMR
CSB-BP478442TMR
CSB-MP478442TMR
CSB-EP478442TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable spermidine synthase (speE) CSB-YP478443TMR
CSB-EP478443TMR
CSB-BP478443TMR
CSB-MP478443TMR
CSB-EP478443TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus DNA repair and recombination protein radB (radB) CSB-YP478444TMR
CSB-EP478444TMR
CSB-BP478444TMR
CSB-MP478444TMR
CSB-EP478444TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable aminomethyltransferase (gcvT) CSB-YP478445TMR
CSB-EP478445TMR
CSB-BP478445TMR
CSB-MP478445TMR
CSB-EP478445TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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