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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Probable cytokinin riboside 5'-monophosphate phosphoribohydrolase LOGL9 (LOGL9) CSB-YP478446OFG
CSB-EP478446OFG
CSB-BP478446OFG
CSB-MP478446OFG
CSB-EP478446OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable sucrose-phosphate synthase 2 (SPS2) CSB-YP478447OFG
CSB-EP478447OFG
CSB-BP478447OFG
CSB-MP478447OFG
CSB-EP478447OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis WD repeat-containing protein on Y chromosome (WDY) CSB-YP478448DMP
CSB-EP478448DMP
CSB-BP478448DMP
CSB-MP478448DMP
CSB-EP478448DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago truncatula Lipoyl synthase, mitochondrial (LIP1) CSB-YP478449MQP
CSB-EP478449MQP
CSB-BP478449MQP
CSB-MP478449MQP
CSB-EP478449MQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Adenylosuccinate synthetase (PHATRDRAFT_26256), partial CSB-YP478450EUF
CSB-EP478450EUF
CSB-BP478450EUF
CSB-MP478450EUF
CSB-EP478450EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaeodactylum tricornutum Translation factor GUF1 homolog, mitochondrial (PHATRDRAFT_22622) CSB-YP478451EUF
CSB-EP478451EUF
CSB-BP478451EUF
CSB-MP478451EUF
CSB-EP478451EUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Cell division protein SepF (sepF) CSB-YP478452BZO
CSB-EP478452BZO
CSB-BP478452BZO
CSB-MP478452BZO
CSB-EP478452BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 50S ribosomal protein L25 (rplY) CSB-YP478453BZO
CSB-EP478453BZO
CSB-BP478453BZO
CSB-MP478453BZO
CSB-EP478453BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Histidine--tRNA ligase (hisS) CSB-YP478454BZO
CSB-EP478454BZO
CSB-BP478454BZO
CSB-MP478454BZO
CSB-EP478454BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP478455BZO
CSB-EP478455BZO
CSB-BP478455BZO
CSB-MP478455BZO
CSB-EP478455BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP478456BZO
CSB-EP478456BZO
CSB-BP478456BZO
CSB-MP478456BZO
CSB-EP478456BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus ATP-dependent protease subunit HslV (hslV) CSB-YP478457BZO
CSB-EP478457BZO
CSB-BP478457BZO
CSB-MP478457BZO
CSB-EP478457BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus tRNA (guanine-N (1)-)-methyltransferase CSB-YP478458BZO
CSB-EP478458BZO
CSB-BP478458BZO
CSB-MP478458BZO
CSB-EP478458BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP478459BZO
CSB-EP478459BZO
CSB-BP478459BZO
CSB-MP478459BZO
CSB-EP478459BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus UPF0348 protein Aflv_1846 (Aflv_1846) CSB-YP478460BZO
CSB-EP478460BZO
CSB-BP478460BZO
CSB-MP478460BZO
CSB-EP478460BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus L-arabinose isomerase (araA) CSB-YP478461BZO
CSB-EP478461BZO
CSB-BP478461BZO
CSB-MP478461BZO
CSB-EP478461BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Trigger factor (tig) CSB-YP478462BZO
CSB-EP478462BZO
CSB-BP478462BZO
CSB-MP478462BZO
CSB-EP478462BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP478463BZO
CSB-EP478463BZO
CSB-BP478463BZO
CSB-MP478463BZO
CSB-EP478463BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Elongation factor P (efp) CSB-YP478464BZO
CSB-EP478464BZO
CSB-BP478464BZO
CSB-MP478464BZO
CSB-EP478464BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Cytidylate kinase (cmk) CSB-YP478465BZO
CSB-EP478465BZO
CSB-BP478465BZO
CSB-MP478465BZO
CSB-EP478465BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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