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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cyanothece sp. 50S ribosomal protein L17 (rplQ) CSB-YP478979EPW
CSB-EP478979EPW
CSB-BP478979EPW
CSB-MP478979EPW
CSB-EP478979EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 30S ribosomal protein S5 (rpsE) CSB-YP478980EPW
CSB-EP478980EPW
CSB-BP478980EPW
CSB-MP478980EPW
CSB-EP478980EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L29 (rpmC) CSB-YP478981EPW
CSB-EP478981EPW
CSB-BP478981EPW
CSB-MP478981EPW
CSB-EP478981EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Histidine--tRNA ligase (hisS) CSB-YP478982EPW
CSB-EP478982EPW
CSB-BP478982EPW
CSB-MP478982EPW
CSB-EP478982EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP478983EPW
CSB-EP478983EPW
CSB-BP478983EPW
CSB-MP478983EPW
CSB-EP478983EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP478984EPW
CSB-EP478984EPW
CSB-BP478984EPW
CSB-MP478984EPW
CSB-EP478984EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L4 (rplD) CSB-YP478985EPW
CSB-EP478985EPW
CSB-BP478985EPW
CSB-MP478985EPW
CSB-EP478985EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. UPF0059 membrane protein PCC8801_2453 (PCC8801_2453), partial CSB-YP478986EPW1
CSB-EP478986EPW1
CSB-BP478986EPW1
CSB-MP478986EPW1
CSB-EP478986EPW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP478987EPW
CSB-EP478987EPW
CSB-BP478987EPW
CSB-MP478987EPW
CSB-EP478987EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP478988EPW
CSB-EP478988EPW
CSB-BP478988EPW
CSB-MP478988EPW
CSB-EP478988EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP478989EPW
CSB-EP478989EPW
CSB-BP478989EPW
CSB-MP478989EPW
CSB-EP478989EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. ATP synthase gamma chain (atpG) CSB-YP478990EPW
CSB-EP478990EPW
CSB-BP478990EPW
CSB-MP478990EPW
CSB-EP478990EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP478991EPW
CSB-EP478991EPW
CSB-BP478991EPW
CSB-MP478991EPW
CSB-EP478991EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Cytochrome b559 subunit beta (psbF), partial CSB-YP478992EPW
CSB-EP478992EPW
CSB-BP478992EPW
CSB-MP478992EPW
CSB-EP478992EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ribose-5-phosphate isomerase A (rpiA) CSB-YP478993EPW
CSB-EP478993EPW
CSB-BP478993EPW
CSB-MP478993EPW
CSB-EP478993EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 7-cyano-7-deazaguanine synthase (queC) CSB-YP478994DZZ
CSB-EP478994DZZ
CSB-BP478994DZZ
CSB-MP478994DZZ
CSB-EP478994DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Protein translocase subunit SecA (secA), partial CSB-YP478995DZZ
CSB-EP478995DZZ
CSB-BP478995DZZ
CSB-MP478995DZZ
CSB-EP478995DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Probable transcriptional regulatory protein PCC7424_2775 (PCC7424_2775) CSB-YP478996DZZ
CSB-EP478996DZZ
CSB-BP478996DZZ
CSB-MP478996DZZ
CSB-EP478996DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Deoxyribose-phosphate aldolase (deoC) CSB-YP478997DZZ
CSB-EP478997DZZ
CSB-BP478997DZZ
CSB-MP478997DZZ
CSB-EP478997DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP478998DZZ
CSB-EP478998DZZ
CSB-BP478998DZZ
CSB-MP478998DZZ
CSB-EP478998DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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