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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cyanothece sp. Cobyric acid synthase (cobQ) CSB-YP478959EPW
CSB-EP478959EPW
CSB-BP478959EPW
CSB-MP478959EPW
CSB-EP478959EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Sugar fermentation stimulation protein homolog (sfsA) CSB-YP478960EPW
CSB-EP478960EPW
CSB-BP478960EPW
CSB-MP478960EPW
CSB-EP478960EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. DNA-directed RNA polymerase subunit gamma (rpoC1), partial CSB-YP478961EPW
CSB-EP478961EPW
CSB-BP478961EPW
CSB-MP478961EPW
CSB-EP478961EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 50S ribosomal protein L11 (rplK) CSB-YP478962EPW
CSB-EP478962EPW
CSB-BP478962EPW
CSB-MP478962EPW
CSB-EP478962EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Aspartate 1-decarboxylase (panD) CSB-YP478963EPW
CSB-EP478963EPW
CSB-BP478963EPW
CSB-MP478963EPW
CSB-EP478963EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Adenylosuccinate synthetase (purA) CSB-YP478964EPW
CSB-EP478964EPW
CSB-BP478964EPW
CSB-MP478964EPW
CSB-EP478964EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Potassium-transporting ATPase A chain (kdpA), partial CSB-YP478965EPW
CSB-EP478965EPW
CSB-BP478965EPW
CSB-MP478965EPW
CSB-EP478965EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Leucine--tRNA ligase (leuS), partial CSB-YP478966EPW
CSB-EP478966EPW
CSB-BP478966EPW
CSB-MP478966EPW
CSB-EP478966EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. NAD (P)H-quinone oxidoreductase subunit N CSB-YP478967EPW
CSB-EP478967EPW
CSB-BP478967EPW
CSB-MP478967EPW
CSB-EP478967EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Hydroxylamine reductase (hcp), partial CSB-YP478968EPW
CSB-EP478968EPW
CSB-BP478968EPW
CSB-MP478968EPW
CSB-EP478968EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. UPF0284 protein PCC8801_3324 (PCC8801_3324) CSB-YP478969EPW
CSB-EP478969EPW
CSB-BP478969EPW
CSB-MP478969EPW
CSB-EP478969EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP478970EPW
CSB-EP478970EPW
CSB-BP478970EPW
CSB-MP478970EPW
CSB-EP478970EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Glutamate 5-kinase (proB) CSB-YP478971EPW
CSB-EP478971EPW
CSB-BP478971EPW
CSB-MP478971EPW
CSB-EP478971EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Peptide chain release factor 2 (prfB) CSB-YP478972EPW
CSB-EP478972EPW
CSB-BP478972EPW
CSB-MP478972EPW
CSB-EP478972EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ketol-acid reductoisomerase (ilvC) CSB-YP478973EPW
CSB-EP478973EPW
CSB-BP478973EPW
CSB-MP478973EPW
CSB-EP478973EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Protein translocase subunit SecA (secA), partial CSB-YP478974EPW
CSB-EP478974EPW
CSB-BP478974EPW
CSB-MP478974EPW
CSB-EP478974EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Diaminopimelate epimerase (dapF) CSB-YP478975EPW
CSB-EP478975EPW
CSB-BP478975EPW
CSB-MP478975EPW
CSB-EP478975EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Proline--tRNA ligase (proS), partial CSB-YP478976EPW
CSB-EP478976EPW
CSB-BP478976EPW
CSB-MP478976EPW
CSB-EP478976EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. GTPase Der (der) CSB-YP478977EPW
CSB-EP478977EPW
CSB-BP478977EPW
CSB-MP478977EPW
CSB-EP478977EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP478978EPW
CSB-EP478978EPW
CSB-BP478978EPW
CSB-MP478978EPW
CSB-EP478978EPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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