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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP478899BQM
CSB-EP478899BQM
CSB-BP478899BQM
CSB-MP478899BQM
CSB-EP478899BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP478900BQM
CSB-EP478900BQM
CSB-BP478900BQM
CSB-MP478900BQM
CSB-EP478900BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP478901BQM
CSB-EP478901BQM
CSB-BP478901BQM
CSB-MP478901BQM
CSB-EP478901BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydrodipicolinate reductase (dapB) CSB-YP478902BQM
CSB-EP478902BQM
CSB-BP478902BQM
CSB-MP478902BQM
CSB-EP478902BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L31 type B (rpmE2) CSB-YP478903BQM
CSB-EP478903BQM
CSB-BP478903BQM
CSB-MP478903BQM
CSB-EP478903BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP478904BQM
CSB-EP478904BQM
CSB-BP478904BQM
CSB-MP478904BQM
CSB-EP478904BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazolonepropionase (hutI) CSB-YP478905BQM
CSB-EP478905BQM
CSB-BP478905BQM
CSB-MP478905BQM
CSB-EP478905BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein hfq (hfq) CSB-YP478906BQM
CSB-EP478906BQM
CSB-BP478906BQM
CSB-MP478906BQM
CSB-EP478906BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Nucleoid occlusion protein (noc) CSB-YP478907BQM
CSB-EP478907BQM
CSB-BP478907BQM
CSB-MP478907BQM
CSB-EP478907BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA mismatch repair protein MutS (mutS), partial CSB-YP478908BQM
CSB-EP478908BQM
CSB-BP478908BQM
CSB-MP478908BQM
CSB-EP478908BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S15 (rpsO) CSB-YP478909BQM
CSB-EP478909BQM
CSB-BP478909BQM
CSB-MP478909BQM
CSB-EP478909BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP478910BQM
CSB-EP478910BQM
CSB-BP478910BQM
CSB-MP478910BQM
CSB-EP478910BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0133 protein BCAH820_0024 (BCAH820_0024) CSB-YP478911BQM
CSB-EP478911BQM
CSB-BP478911BQM
CSB-MP478911BQM
CSB-EP478911BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP478912BQM
CSB-EP478912BQM
CSB-BP478912BQM
CSB-MP478912BQM
CSB-EP478912BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA (guanine-N (1)-)-methyltransferase CSB-YP478913BQM
CSB-EP478913BQM
CSB-BP478913BQM
CSB-MP478913BQM
CSB-EP478913BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L28 (rpmB) CSB-YP478914BQM
CSB-EP478914BQM
CSB-BP478914BQM
CSB-MP478914BQM
CSB-EP478914BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP478915BQM
CSB-EP478915BQM
CSB-BP478915BQM
CSB-MP478915BQM
CSB-EP478915BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0747 protein BCAH820_3934 (BCAH820_3934), partial CSB-YP478916BQM
CSB-EP478916BQM
CSB-BP478916BQM
CSB-MP478916BQM
CSB-EP478916BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0348 protein BCAH820_3940 (BCAH820_3940) CSB-YP478917BQM
CSB-EP478917BQM
CSB-BP478917BQM
CSB-MP478917BQM
CSB-EP478917BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative septation protein spoVG (spoVG) CSB-YP478918BQM
CSB-EP478918BQM
CSB-BP478918BQM
CSB-MP478918BQM
CSB-EP478918BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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