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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella heidelberg UPF0102 protein yraN (yraN) CSB-YP479226SWQ
CSB-EP479226SWQ
CSB-BP479226SWQ
CSB-MP479226SWQ
CSB-EP479226SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Ribosome maturation factor RimP (rimP) CSB-YP479227SWQ
CSB-EP479227SWQ
CSB-BP479227SWQ
CSB-MP479227SWQ
CSB-EP479227SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Ribulokinase (araB), partial CSB-YP479228SWQ
CSB-EP479228SWQ
CSB-BP479228SWQ
CSB-MP479228SWQ
CSB-EP479228SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP479229SWQ
CSB-EP479229SWQ
CSB-BP479229SWQ
CSB-MP479229SWQ
CSB-EP479229SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 50S ribosomal protein L13 (rplM) CSB-YP479230SWQ
CSB-EP479230SWQ
CSB-BP479230SWQ
CSB-MP479230SWQ
CSB-EP479230SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg DNA-binding protein fis (fis) CSB-YP479231SWQ
CSB-EP479231SWQ
CSB-BP479231SWQ
CSB-MP479231SWQ
CSB-EP479231SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 30S ribosomal protein S11 (rpsK) CSB-YP479232SWQ
CSB-EP479232SWQ
CSB-BP479232SWQ
CSB-MP479232SWQ
CSB-EP479232SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 50S ribosomal protein L6 (rplF) CSB-YP479233SWQ
CSB-EP479233SWQ
CSB-BP479233SWQ
CSB-MP479233SWQ
CSB-EP479233SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP479234SWQ
CSB-EP479234SWQ
CSB-BP479234SWQ
CSB-MP479234SWQ
CSB-EP479234SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP479235SWQ
CSB-EP479235SWQ
CSB-BP479235SWQ
CSB-MP479235SWQ
CSB-EP479235SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP479236SWQ
CSB-EP479236SWQ
CSB-BP479236SWQ
CSB-MP479236SWQ
CSB-EP479236SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0260 protein ycgN (ycgN) CSB-YP479237SWQ
CSB-EP479237SWQ
CSB-BP479237SWQ
CSB-MP479237SWQ
CSB-EP479237SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg UPF0059 membrane protein yebN (yebN), partial CSB-YP479238SWQ1
CSB-EP479238SWQ1
CSB-BP479238SWQ1
CSB-MP479238SWQ1
CSB-EP479238SWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 50S ribosomal protein L14 (rplN) CSB-YP479239SWQ
CSB-EP479239SWQ
CSB-BP479239SWQ
CSB-MP479239SWQ
CSB-EP479239SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 50S ribosomal protein L22 (rplV) CSB-YP479240SWQ
CSB-EP479240SWQ
CSB-BP479240SWQ
CSB-MP479240SWQ
CSB-EP479240SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg 30S ribosomal protein S12 (rpsL) CSB-YP479241SWQ
CSB-EP479241SWQ
CSB-BP479241SWQ
CSB-MP479241SWQ
CSB-EP479241SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg Ferrous iron transport protein C (feoC) CSB-YP479242SWQ
CSB-EP479242SWQ
CSB-BP479242SWQ
CSB-MP479242SWQ
CSB-EP479242SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Transcriptional repressor NrdR (nrdR) CSB-YP479243SWV
CSB-EP479243SWV
CSB-BP479243SWV
CSB-MP479243SWV
CSB-EP479243SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0234 protein yajQ (yajQ) CSB-YP479244SWV
CSB-EP479244SWV
CSB-BP479244SWV
CSB-MP479244SWV
CSB-EP479244SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Putative transport protein YidE (yidE), partial CSB-YP479245SWV
CSB-EP479245SWV
CSB-BP479245SWV
CSB-MP479245SWV
CSB-EP479245SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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