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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella newport Thiosulfate sulfurtransferase glpE (glpE) CSB-YP480079SWS
CSB-EP480079SWS
CSB-BP480079SWS
CSB-MP480079SWS
CSB-EP480079SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Xanthine phosphoribosyltransferase (gpt) CSB-YP480080SWS
CSB-EP480080SWS
CSB-BP480080SWS
CSB-MP480080SWS
CSB-EP480080SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0178 protein yaiI (yaiI) CSB-YP480081SWS
CSB-EP480081SWS
CSB-BP480081SWS
CSB-MP480081SWS
CSB-EP480081SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0345 protein YaiE (yaiE) CSB-YP480082SWS
CSB-EP480082SWS
CSB-BP480082SWS
CSB-MP480082SWS
CSB-EP480082SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP480083SWS
CSB-EP480083SWS
CSB-BP480083SWS
CSB-MP480083SWS
CSB-EP480083SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 2,3-diketo-L-gulonate reductase (dlgD) CSB-YP480084SWS
CSB-EP480084SWS
CSB-BP480084SWS
CSB-MP480084SWS
CSB-EP480084SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Recombination-associated protein rdgC (rdgC) CSB-YP480085SWS
CSB-EP480085SWS
CSB-BP480085SWS
CSB-MP480085SWS
CSB-EP480085SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP480086SWS
CSB-EP480086SWS
CSB-BP480086SWS
CSB-MP480086SWS
CSB-EP480086SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP480087SWS
CSB-EP480087SWS
CSB-BP480087SWS
CSB-MP480087SWS
CSB-EP480087SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Cobalt transport protein CbiN (cbiN), partial CSB-YP480088SWS1
CSB-EP480088SWS1
CSB-BP480088SWS1
CSB-MP480088SWS1
CSB-EP480088SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP480089SWS
CSB-EP480089SWS
CSB-BP480089SWS
CSB-MP480089SWS
CSB-EP480089SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP480090SWS
CSB-EP480090SWS
CSB-BP480090SWS
CSB-MP480090SWS
CSB-EP480090SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP480091SWS
CSB-EP480091SWS
CSB-BP480091SWS
CSB-MP480091SWS
CSB-EP480091SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Allantoinase (allB) CSB-YP480092SWS
CSB-EP480092SWS
CSB-BP480092SWS
CSB-MP480092SWS
CSB-EP480092SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP480093SWS
CSB-EP480093SWS
CSB-BP480093SWS
CSB-MP480093SWS
CSB-EP480093SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport D-serine dehydratase (dsdA) CSB-YP480094SWS
CSB-EP480094SWS
CSB-BP480094SWS
CSB-MP480094SWS
CSB-EP480094SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Ribonuclease P protein component (rnpA) CSB-YP480095SWS
CSB-EP480095SWS
CSB-BP480095SWS
CSB-MP480095SWS
CSB-EP480095SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport ATP synthase subunit c (atpE), partial CSB-YP480096SWS1
CSB-EP480096SWS1
CSB-BP480096SWS1
CSB-MP480096SWS1
CSB-EP480096SWS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Low affinity potassium transport system protein kup (kup), partial CSB-YP480097SWS
CSB-EP480097SWS
CSB-BP480097SWS
CSB-MP480097SWS
CSB-EP480097SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport UPF0250 protein ybeD (ybeD) CSB-YP480098SWS
CSB-EP480098SWS
CSB-BP480098SWS
CSB-MP480098SWS
CSB-EP480098SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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