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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Salmonella dublin UPF0102 protein yraN (yraN) CSB-YP480679SWN
CSB-EP480679SWN
CSB-BP480679SWN
CSB-MP480679SWN
CSB-EP480679SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosome maturation factor RimP (rimP) CSB-YP480680SWN
CSB-EP480680SWN
CSB-BP480680SWN
CSB-MP480680SWN
CSB-EP480680SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin RNA polymerase-associated protein RapA (rapA), partial CSB-YP480681SWN
CSB-EP480681SWN
CSB-BP480681SWN
CSB-MP480681SWN
CSB-EP480681SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP480682SWN
CSB-EP480682SWN
CSB-BP480682SWN
CSB-MP480682SWN
CSB-EP480682SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0289 protein yacF (yacF) CSB-YP480683SWN
CSB-EP480683SWN
CSB-BP480683SWN
CSB-MP480683SWN
CSB-EP480683SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Putative sialic acid transporter (nanT), partial CSB-YP480684SWN
CSB-EP480684SWN
CSB-BP480684SWN
CSB-MP480684SWN
CSB-EP480684SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP480685SWN1
CSB-EP480685SWN1
CSB-BP480685SWN1
CSB-MP480685SWN1
CSB-EP480685SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP480686SWN
CSB-EP480686SWN
CSB-BP480686SWN
CSB-MP480686SWN
CSB-EP480686SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Iron--sulfur cluster insertion protein ErpA (erpA) CSB-YP480687SWN
CSB-EP480687SWN
CSB-BP480687SWN
CSB-MP480687SWN
CSB-EP480687SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribonuclease HII (rnhB) CSB-YP480688SWN
CSB-EP480688SWN
CSB-BP480688SWN
CSB-MP480688SWN
CSB-EP480688SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0294 protein yafD (yafD) CSB-YP480689SWN
CSB-EP480689SWN
CSB-BP480689SWN
CSB-MP480689SWN
CSB-EP480689SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Vitamin B12 import system permease protein BtuC (btuC), partial CSB-YP480690SWN
CSB-EP480690SWN
CSB-BP480690SWN
CSB-MP480690SWN
CSB-EP480690SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin NH (3)-dependent NAD (+) synthetase CSB-YP480691SWN
CSB-EP480691SWN
CSB-BP480691SWN
CSB-MP480691SWN
CSB-EP480691SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Protein smg (smg) CSB-YP480692SWN
CSB-EP480692SWN
CSB-BP480692SWN
CSB-MP480692SWN
CSB-EP480692SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L17 (rplQ) CSB-YP480693SWN
CSB-EP480693SWN
CSB-BP480693SWN
CSB-MP480693SWN
CSB-EP480693SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 30S ribosomal protein S14 (rpsN) CSB-YP480694SWN
CSB-EP480694SWN
CSB-BP480694SWN
CSB-MP480694SWN
CSB-EP480694SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L22 (rplV) CSB-YP480695SWN
CSB-EP480695SWN
CSB-BP480695SWN
CSB-MP480695SWN
CSB-EP480695SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 50S ribosomal protein L3 (rplC) CSB-YP480696SWN
CSB-EP480696SWN
CSB-BP480696SWN
CSB-MP480696SWN
CSB-EP480696SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Siroheme synthase (cysG) CSB-YP480697SWN
CSB-EP480697SWN
CSB-BP480697SWN
CSB-MP480697SWN
CSB-EP480697SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin High frequency lysogenization protein HflD homolog (hflD) CSB-YP480698SWN
CSB-EP480698SWN
CSB-BP480698SWN
CSB-MP480698SWN
CSB-EP480698SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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