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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Escherichia coli O157:H7 Zinc transporter ZupT (zupT), partial CSB-YP481159EOE1
CSB-EP481159EOE1
CSB-BP481159EOE1
CSB-MP481159EOE1
CSB-EP481159EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP481160EOE
CSB-EP481160EOE
CSB-BP481160EOE
CSB-MP481160EOE
CSB-EP481160EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Undecaprenyl-diphosphatase (uppP), partial CSB-YP481161EOE1
CSB-EP481161EOE1
CSB-BP481161EOE1
CSB-MP481161EOE1
CSB-EP481161EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 8-amino-7-oxononanoate synthase (bioF) CSB-YP481162EOE
CSB-EP481162EOE
CSB-BP481162EOE
CSB-MP481162EOE
CSB-EP481162EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Flagellar protein FliT (fliT) CSB-YP481163EOE
CSB-EP481163EOE
CSB-BP481163EOE
CSB-MP481163EOE
CSB-EP481163EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 5-keto-4-deoxy-D-glucarate aldolase (garL) CSB-YP481164EOE
CSB-EP481164EOE
CSB-BP481164EOE
CSB-MP481164EOE
CSB-EP481164EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L21 (rplU) CSB-YP481165EOE
CSB-EP481165EOE
CSB-BP481165EOE
CSB-MP481165EOE
CSB-EP481165EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Hydroxylamine reductase (hcp) CSB-YP481166EOE
CSB-EP481166EOE
CSB-BP481166EOE
CSB-MP481166EOE
CSB-EP481166EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP481167EOE
CSB-EP481167EOE
CSB-BP481167EOE
CSB-MP481167EOE
CSB-EP481167EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Arginine repressor (argR) CSB-YP481168EOE
CSB-EP481168EOE
CSB-BP481168EOE
CSB-MP481168EOE
CSB-EP481168EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Shikimate dehydrogenase (aroE) CSB-YP481169EOE
CSB-EP481169EOE
CSB-BP481169EOE
CSB-MP481169EOE
CSB-EP481169EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S11 (rpsK) CSB-YP481170EOE
CSB-EP481170EOE
CSB-BP481170EOE
CSB-MP481170EOE
CSB-EP481170EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP481171EOE
CSB-EP481171EOE
CSB-BP481171EOE
CSB-MP481171EOE
CSB-EP481171EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0434 protein ycaR (ycaR) CSB-YP481172EOE
CSB-EP481172EOE
CSB-BP481172EOE
CSB-MP481172EOE
CSB-EP481172EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Dihydroorotate dehydrogenase (quinone) CSB-YP481173EOE
CSB-EP481173EOE
CSB-BP481173EOE
CSB-MP481173EOE
CSB-EP481173EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L15 (rplO) CSB-YP481174EOE
CSB-EP481174EOE
CSB-BP481174EOE
CSB-MP481174EOE
CSB-EP481174EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S14 (rpsN) CSB-YP481175EOE
CSB-EP481175EOE
CSB-BP481175EOE
CSB-MP481175EOE
CSB-EP481175EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L16 (rplP) CSB-YP481176EOE
CSB-EP481176EOE
CSB-BP481176EOE
CSB-MP481176EOE
CSB-EP481176EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L3 (rplC) CSB-YP481177EOE
CSB-EP481177EOE
CSB-BP481177EOE
CSB-MP481177EOE
CSB-EP481177EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein TusC (tusC) CSB-YP481178EOE
CSB-EP481178EOE
CSB-BP481178EOE
CSB-MP481178EOE
CSB-EP481178EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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