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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Anaeromyxobacter sp. Protein FdhD homolog (fdhD) CSB-YP480359BYT
CSB-EP480359BYT
CSB-BP480359BYT
CSB-MP480359BYT
CSB-EP480359BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP480360BYT
CSB-EP480360BYT
CSB-BP480360BYT
CSB-MP480360BYT
CSB-EP480360BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Cysteine--tRNA ligase (cysS) CSB-YP480361BYT
CSB-EP480361BYT
CSB-BP480361BYT
CSB-MP480361BYT
CSB-EP480361BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP480362BYT
CSB-EP480362BYT
CSB-BP480362BYT
CSB-MP480362BYT
CSB-EP480362BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP480363BYT
CSB-EP480363BYT
CSB-BP480363BYT
CSB-MP480363BYT
CSB-EP480363BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Anthranilate phosphoribosyltransferase (trpD) CSB-YP480364BYT
CSB-EP480364BYT
CSB-BP480364BYT
CSB-MP480364BYT
CSB-EP480364BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Ribosome-binding factor A (rbfA) CSB-YP480365BYT
CSB-EP480365BYT
CSB-BP480365BYT
CSB-MP480365BYT
CSB-EP480365BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Dihydrodipicolinate synthase (dapA) CSB-YP480366BYT
CSB-EP480366BYT
CSB-BP480366BYT
CSB-MP480366BYT
CSB-EP480366BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. NADH-quinone oxidoreductase subunit D 2 (nuoD2) CSB-YP480367BYT
CSB-EP480367BYT
CSB-BP480367BYT
CSB-MP480367BYT
CSB-EP480367BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Probable DNA ligase (lig), partial CSB-YP480368BYT
CSB-EP480368BYT
CSB-BP480368BYT
CSB-MP480368BYT
CSB-EP480368BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP480369BYT
CSB-EP480369BYT
CSB-BP480369BYT
CSB-MP480369BYT
CSB-EP480369BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Maf-like protein AnaeK_1353 (AnaeK_1353) CSB-YP480370BYT
CSB-EP480370BYT
CSB-BP480370BYT
CSB-MP480370BYT
CSB-EP480370BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Type III pantothenate kinase (coaX) CSB-YP480371BYT
CSB-EP480371BYT
CSB-BP480371BYT
CSB-MP480371BYT
CSB-EP480371BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP480372BYT
CSB-EP480372BYT
CSB-BP480372BYT
CSB-MP480372BYT
CSB-EP480372BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 50S ribosomal protein L9 (rplI) CSB-YP480373BYT
CSB-EP480373BYT
CSB-BP480373BYT
CSB-MP480373BYT
CSB-EP480373BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. Undecaprenyl-diphosphatase (uppP), partial CSB-YP480374BYT1
CSB-EP480374BYT1
CSB-BP480374BYT1
CSB-MP480374BYT1
CSB-EP480374BYT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter sp. 30S ribosomal protein S2 (rpsB) CSB-YP480375BYT
CSB-EP480375BYT
CSB-BP480375BYT
CSB-MP480375BYT
CSB-EP480375BYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP480376IMT
CSB-EP480376IMT
CSB-BP480376IMT
CSB-MP480376IMT
CSB-EP480376IMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina C-type lectin A12 CSB-YP480377MPJ
CSB-EP480377MPJ
CSB-BP480377MPJ
CSB-MP480377MPJ
CSB-EP480377MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina C-type lectin A4 CSB-YP480378MPJ
CSB-EP480378MPJ
CSB-BP480378MPJ
CSB-MP480378MPJ
CSB-EP480378MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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