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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli L-rhamnose isomerase (rhaA) CSB-YP481480ENW
CSB-EP481480ENW
CSB-BP481480ENW
CSB-MP481480ENW
CSB-EP481480ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Regulator of ribonuclease activity A (rraA) CSB-YP481481ENW
CSB-EP481481ENW
CSB-BP481481ENW
CSB-MP481481ENW
CSB-EP481481ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cell division protein ZipA homolog (zipA), partial CSB-YP481482ENW
CSB-EP481482ENW
CSB-BP481482ENW
CSB-MP481482ENW
CSB-EP481482ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP481483ENW
CSB-EP481483ENW
CSB-BP481483ENW
CSB-MP481483ENW
CSB-EP481483ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DnaA-homolog protein hda (hda) CSB-YP481484ENW
CSB-EP481484ENW
CSB-BP481484ENW
CSB-MP481484ENW
CSB-EP481484ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli GTPase Der (der) CSB-YP481485ENW
CSB-EP481485ENW
CSB-BP481485ENW
CSB-MP481485ENW
CSB-EP481485ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Co-chaperone protein hscB (hscB) CSB-YP481486ENW
CSB-EP481486ENW
CSB-BP481486ENW
CSB-MP481486ENW
CSB-EP481486ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD (+) reductase component CSB-YP481487ENW
CSB-EP481487ENW
CSB-BP481487ENW
CSB-MP481487ENW
CSB-EP481487ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acetylglutamate kinase (argB) CSB-YP481488ENW
CSB-EP481488ENW
CSB-BP481488ENW
CSB-MP481488ENW
CSB-EP481488ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L1 (rplA) CSB-YP481489ENW
CSB-EP481489ENW
CSB-BP481489ENW
CSB-MP481489ENW
CSB-EP481489ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Thiamine-phosphate synthase (thiE) CSB-YP481490ENW
CSB-EP481490ENW
CSB-BP481490ENW
CSB-MP481490ENW
CSB-EP481490ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NADH pyrophosphatase (nudC) CSB-YP481491ENW
CSB-EP481491ENW
CSB-BP481491ENW
CSB-MP481491ENW
CSB-EP481491ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Maltoporin (lamB) CSB-YP481492ENW
CSB-EP481492ENW
CSB-BP481492ENW
CSB-MP481492ENW
CSB-EP481492ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cytochrome c-552 (nrfA) CSB-YP481493ENW
CSB-EP481493ENW
CSB-BP481493ENW
CSB-MP481493ENW
CSB-EP481493ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 60 kDa chaperonin (groL) CSB-YP481494ENW
CSB-EP481494ENW
CSB-BP481494ENW
CSB-MP481494ENW
CSB-EP481494ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein grpE (grpE) CSB-YP481495ENW
CSB-EP481495ENW
CSB-BP481495ENW
CSB-MP481495ENW
CSB-EP481495ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli S-ribosylhomocysteine lyase (luxS) CSB-YP481496ENW
CSB-EP481496ENW
CSB-BP481496ENW
CSB-MP481496ENW
CSB-EP481496ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nitric oxide reductase FlRd-NAD (+) reductase CSB-YP481497ENW
CSB-EP481497ENW
CSB-BP481497ENW
CSB-MP481497ENW
CSB-EP481497ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP481498ENW
CSB-EP481498ENW
CSB-BP481498ENW
CSB-MP481498ENW
CSB-EP481498ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-fucose isomerase (fucI) CSB-YP481499ENW
CSB-EP481499ENW
CSB-BP481499ENW
CSB-MP481499ENW
CSB-EP481499ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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