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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae serotype 19F D-alanine--D-alanine ligase (ddl) CSB-YP480499FNE
CSB-EP480499FNE
CSB-BP480499FNE
CSB-MP480499FNE
CSB-EP480499FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Ribonuclease HIII (rnhC) CSB-YP480500FNE
CSB-EP480500FNE
CSB-BP480500FNE
CSB-MP480500FNE
CSB-EP480500FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Anthranilate phosphoribosyltransferase (trpD) CSB-YP480501FNE
CSB-EP480501FNE
CSB-BP480501FNE
CSB-MP480501FNE
CSB-EP480501FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP480502GFN
CSB-EP480502GFN
CSB-BP480502GFN
CSB-MP480502GFN
CSB-EP480502GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP480503GFN
CSB-EP480503GFN
CSB-BP480503GFN
CSB-MP480503GFN
CSB-EP480503GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis GTPase obg (obg) CSB-YP480504GFN
CSB-EP480504GFN
CSB-BP480504GFN
CSB-MP480504GFN
CSB-EP480504GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP480505GFN
CSB-EP480505GFN
CSB-BP480505GFN
CSB-MP480505GFN
CSB-EP480505GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP480506GFN
CSB-EP480506GFN
CSB-BP480506GFN
CSB-MP480506GFN
CSB-EP480506GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Bifunctional protein pyrR (pyrR) CSB-YP480507GFN
CSB-EP480507GFN
CSB-BP480507GFN
CSB-MP480507GFN
CSB-EP480507GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L19 (rplS) CSB-YP480508GFN
CSB-EP480508GFN
CSB-BP480508GFN
CSB-MP480508GFN
CSB-EP480508GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP480509GFN
CSB-EP480509GFN
CSB-BP480509GFN
CSB-MP480509GFN
CSB-EP480509GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP480510GFN
CSB-EP480510GFN
CSB-BP480510GFN
CSB-MP480510GFN
CSB-EP480510GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Adenylosuccinate synthetase (purA) CSB-YP480511GFN
CSB-EP480511GFN
CSB-BP480511GFN
CSB-MP480511GFN
CSB-EP480511GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP480512GFN
CSB-EP480512GFN
CSB-BP480512GFN
CSB-MP480512GFN
CSB-EP480512GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP480513GFN
CSB-EP480513GFN
CSB-BP480513GFN
CSB-MP480513GFN
CSB-EP480513GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Leucine--tRNA ligase (leuS), partial CSB-YP480514GFN
CSB-EP480514GFN
CSB-BP480514GFN
CSB-MP480514GFN
CSB-EP480514GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP480515GFN
CSB-EP480515GFN
CSB-BP480515GFN
CSB-MP480515GFN
CSB-EP480515GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Phosphoglycerate kinase (pgk) CSB-YP480516GFN
CSB-EP480516GFN
CSB-BP480516GFN
CSB-MP480516GFN
CSB-EP480516GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP480517GFN
CSB-EP480517GFN
CSB-BP480517GFN
CSB-MP480517GFN
CSB-EP480517GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L4 (rplD) CSB-YP480518GFN
CSB-EP480518GFN
CSB-BP480518GFN
CSB-MP480518GFN
CSB-EP480518GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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