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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter bemidjiensis 50S ribosomal protein L16 (rplP) CSB-YP480519GFN
CSB-EP480519GFN
CSB-BP480519GFN
CSB-MP480519GFN
CSB-EP480519GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP480520GFN
CSB-EP480520GFN
CSB-BP480520GFN
CSB-MP480520GFN
CSB-EP480520GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Adenylate kinase (adk) CSB-YP480521GFN
CSB-EP480521GFN
CSB-BP480521GFN
CSB-MP480521GFN
CSB-EP480521GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP480522GFN
CSB-EP480522GFN
CSB-BP480522GFN
CSB-MP480522GFN
CSB-EP480522GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Dihydrodipicolinate synthase (dapA) CSB-YP480523GFN
CSB-EP480523GFN
CSB-BP480523GFN
CSB-MP480523GFN
CSB-EP480523GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis 50S ribosomal protein L25 (rplY) CSB-YP480524GFN
CSB-EP480524GFN
CSB-BP480524GFN
CSB-MP480524GFN
CSB-EP480524GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP480525GFN
CSB-EP480525GFN
CSB-BP480525GFN
CSB-MP480525GFN
CSB-EP480525GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis tRNA (cmo5U34)-methyltransferase CSB-YP480526GFN
CSB-EP480526GFN
CSB-BP480526GFN
CSB-MP480526GFN
CSB-EP480526GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Tryptophan synthase alpha chain (trpA) CSB-YP480527AWI
CSB-EP480527AWI
CSB-BP480527AWI
CSB-MP480527AWI
CSB-EP480527AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP480528AWI
CSB-EP480528AWI
CSB-BP480528AWI
CSB-MP480528AWI
CSB-EP480528AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP480529AWI
CSB-EP480529AWI
CSB-BP480529AWI
CSB-MP480529AWI
CSB-EP480529AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Biotin synthase (bioB) CSB-YP480530AWI
CSB-EP480530AWI
CSB-BP480530AWI
CSB-MP480530AWI
CSB-EP480530AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S7 (rpsG) CSB-YP480531AWI
CSB-EP480531AWI
CSB-BP480531AWI
CSB-MP480531AWI
CSB-EP480531AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 50S ribosomal protein L22 (rplV) CSB-YP480532AWI
CSB-EP480532AWI
CSB-BP480532AWI
CSB-MP480532AWI
CSB-EP480532AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 30S ribosomal protein S5 (rpsE) CSB-YP480533AWI
CSB-EP480533AWI
CSB-BP480533AWI
CSB-MP480533AWI
CSB-EP480533AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP480534AWI
CSB-EP480534AWI
CSB-BP480534AWI
CSB-MP480534AWI
CSB-EP480534AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP480535AWI
CSB-EP480535AWI
CSB-BP480535AWI
CSB-MP480535AWI
CSB-EP480535AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans 10 kDa chaperonin (groS) CSB-YP480536AWI
CSB-EP480536AWI
CSB-BP480536AWI
CSB-MP480536AWI
CSB-EP480536AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP480537AWI
CSB-EP480537AWI
CSB-BP480537AWI
CSB-MP480537AWI
CSB-EP480537AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP480538AWI1
CSB-EP480538AWI1
CSB-BP480538AWI1
CSB-MP480538AWI1
CSB-EP480538AWI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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