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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella dublin UPF0227 protein ycfP (ycfP) CSB-YP480699SWN
CSB-EP480699SWN
CSB-BP480699SWN
CSB-MP480699SWN
CSB-EP480699SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Phosphate acyltransferase (plsX) CSB-YP480700SWN
CSB-EP480700SWN
CSB-BP480700SWN
CSB-MP480700SWN
CSB-EP480700SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Thiosulfate sulfurtransferase glpE (glpE) CSB-YP480701SWN
CSB-EP480701SWN
CSB-BP480701SWN
CSB-MP480701SWN
CSB-EP480701SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP480702SWN
CSB-EP480702SWN
CSB-BP480702SWN
CSB-MP480702SWN
CSB-EP480702SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin N utilization substance protein B homolog (nusB) CSB-YP480703SWN
CSB-EP480703SWN
CSB-BP480703SWN
CSB-MP480703SWN
CSB-EP480703SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Trigger factor (tig) CSB-YP480704SWN
CSB-EP480704SWN
CSB-BP480704SWN
CSB-MP480704SWN
CSB-EP480704SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0176 protein yceA (yceA) CSB-YP480705SWN
CSB-EP480705SWN
CSB-BP480705SWN
CSB-MP480705SWN
CSB-EP480705SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 2,3-diketo-L-gulonate reductase (dlgD) CSB-YP480706SWN
CSB-EP480706SWN
CSB-BP480706SWN
CSB-MP480706SWN
CSB-EP480706SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Acetyl esterase (aes) CSB-YP480707SWN
CSB-EP480707SWN
CSB-BP480707SWN
CSB-MP480707SWN
CSB-EP480707SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Cobalamin synthase (cobS), partial CSB-YP480708SWN1
CSB-EP480708SWN1
CSB-BP480708SWN1
CSB-MP480708SWN1
CSB-EP480708SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP480709SWN
CSB-EP480709SWN
CSB-BP480709SWN
CSB-MP480709SWN
CSB-EP480709SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Orotate phosphoribosyltransferase (pyrE) CSB-YP480710SWN
CSB-EP480710SWN
CSB-BP480710SWN
CSB-MP480710SWN
CSB-EP480710SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Small heat shock protein ibpB (ibpB) CSB-YP480711SWN
CSB-EP480711SWN
CSB-BP480711SWN
CSB-MP480711SWN
CSB-EP480711SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin LPS-assembly lipoprotein lptE (lptE) CSB-YP480712SWN
CSB-EP480712SWN
CSB-BP480712SWN
CSB-MP480712SWN
CSB-EP480712SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0299 membrane protein yohJ (yohJ), partial CSB-YP480713SWN1
CSB-EP480713SWN1
CSB-BP480713SWN1
CSB-MP480713SWN1
CSB-EP480713SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Chromosomal replication initiator protein DnaA (dnaA) CSB-YP480714SWN
CSB-EP480714SWN
CSB-BP480714SWN
CSB-MP480714SWN
CSB-EP480714SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin ATP synthase subunit c (atpE), partial CSB-YP480715SWN1
CSB-EP480715SWN1
CSB-BP480715SWN1
CSB-MP480715SWN1
CSB-EP480715SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin D-ribose pyranase (rbsD) CSB-YP480716SWN
CSB-EP480716SWN
CSB-BP480716SWN
CSB-MP480716SWN
CSB-EP480716SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin ATP-dependent RNA helicase rhlB (rhlB) CSB-YP480717SWN
CSB-EP480717SWN
CSB-BP480717SWN
CSB-MP480717SWN
CSB-EP480717SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin UPF0271 protein YbgL (ybgL) CSB-YP480718SWN
CSB-EP480718SWN
CSB-BP480718SWN
CSB-MP480718SWN
CSB-EP480718SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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