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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Protein hfq (hfq) CSB-YP481460ENW
CSB-EP481460ENW
CSB-BP481460ENW
CSB-MP481460ENW
CSB-EP481460ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable L-ascorbate-6-phosphate lactonase ulaG (ulaG) CSB-YP481461ENW
CSB-EP481461ENW
CSB-BP481461ENW
CSB-MP481461ENW
CSB-EP481461ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S18 (rpsR) CSB-YP481462ENW
CSB-EP481462ENW
CSB-BP481462ENW
CSB-MP481462ENW
CSB-EP481462ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP481463ENW
CSB-EP481463ENW
CSB-BP481463ENW
CSB-MP481463ENW
CSB-EP481463ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable RNA 2'-phosphotransferase (kptA) CSB-YP481464ENW
CSB-EP481464ENW
CSB-BP481464ENW
CSB-MP481464ENW
CSB-EP481464ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0391 membrane protein ytjA (ytjA), partial CSB-YP481465ENW
CSB-EP481465ENW
CSB-BP481465ENW
CSB-MP481465ENW
CSB-EP481465ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fe/S biogenesis protein NfuA (nfuA) CSB-YP481466ENW
CSB-EP481466ENW
CSB-BP481466ENW
CSB-MP481466ENW
CSB-EP481466ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP481467ENW
CSB-EP481467ENW
CSB-BP481467ENW
CSB-MP481467ENW
CSB-EP481467ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP481468ENW
CSB-EP481468ENW
CSB-BP481468ENW
CSB-MP481468ENW
CSB-EP481468ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L33 (rpmG) CSB-YP481469ENW
CSB-EP481469ENW
CSB-BP481469ENW
CSB-MP481469ENW
CSB-EP481469ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Adenine deaminase (ade) CSB-YP481470ENW
CSB-EP481470ENW
CSB-BP481470ENW
CSB-MP481470ENW
CSB-EP481470ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA replication and repair protein recF (recF) CSB-YP481471ENW
CSB-EP481471ENW
CSB-BP481471ENW
CSB-MP481471ENW
CSB-EP481471ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator yidZ (yidZ) CSB-YP481472ENW
CSB-EP481472ENW
CSB-BP481472ENW
CSB-MP481472ENW
CSB-EP481472ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP synthase subunit c (atpE), partial CSB-YP481473ENW1
CSB-EP481473ENW1
CSB-BP481473ENW1
CSB-MP481473ENW1
CSB-EP481473ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Low affinity potassium transport system protein kup (kup), partial CSB-YP481474ENW
CSB-EP481474ENW
CSB-BP481474ENW
CSB-MP481474ENW
CSB-EP481474ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP481475ENW
CSB-EP481475ENW
CSB-BP481475ENW
CSB-MP481475ENW
CSB-EP481475ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator hdfR (hdfR) CSB-YP481476ENW
CSB-EP481476ENW
CSB-BP481476ENW
CSB-MP481476ENW
CSB-EP481476ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative ECA polymerase (wzyE), partial CSB-YP481477ENW
CSB-EP481477ENW
CSB-BP481477ENW
CSB-MP481477ENW
CSB-EP481477ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP481478ENW
CSB-EP481478ENW
CSB-BP481478ENW
CSB-MP481478ENW
CSB-EP481478ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-tyrosyl-tRNA (Tyr) deacylase CSB-YP481479ENW
CSB-EP481479ENW
CSB-BP481479ENW
CSB-MP481479ENW
CSB-EP481479ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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