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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP482080BQP
CSB-EP482080BQP
CSB-BP482080BQP
CSB-MP482080BQP
CSB-EP482080BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP482081BQP
CSB-EP482081BQP
CSB-BP482081BQP
CSB-MP482081BQP
CSB-EP482081BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0346 protein BCG9842_B3040 (BCG9842_B3040) CSB-YP482082BQP
CSB-EP482082BQP
CSB-BP482082BQP
CSB-MP482082BQP
CSB-EP482082BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Formamidase (amiF) CSB-YP482083BQP
CSB-EP482083BQP
CSB-BP482083BQP
CSB-MP482083BQP
CSB-EP482083BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0358 protein BCG9842_B1188 (BCG9842_B1188) CSB-YP482084BQP
CSB-EP482084BQP
CSB-BP482084BQP
CSB-MP482084BQP
CSB-EP482084BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0356 protein BCG9842_B1161 (BCG9842_B1161) CSB-YP482085BQP
CSB-EP482085BQP
CSB-BP482085BQP
CSB-MP482085BQP
CSB-EP482085BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP482086BQP
CSB-EP482086BQP
CSB-BP482086BQP
CSB-MP482086BQP
CSB-EP482086BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylthioribose kinase (mtnK) CSB-YP482087BQP
CSB-EP482087BQP
CSB-BP482087BQP
CSB-MP482087BQP
CSB-EP482087BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Segregation and condensation protein B (scpB) CSB-YP482088BQP
CSB-EP482088BQP
CSB-BP482088BQP
CSB-MP482088BQP
CSB-EP482088BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP482089BQP
CSB-EP482089BQP
CSB-BP482089BQP
CSB-MP482089BQP
CSB-EP482089BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Acetylglutamate kinase (argB) CSB-YP482090BQP
CSB-EP482090BQP
CSB-BP482090BQP
CSB-MP482090BQP
CSB-EP482090BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP482091BQP
CSB-EP482091BQP
CSB-BP482091BQP
CSB-MP482091BQP
CSB-EP482091BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Shikimate kinase (aroK) CSB-YP482092BQP
CSB-EP482092BQP
CSB-BP482092BQP
CSB-MP482092BQP
CSB-EP482092BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable endonuclease 4 (nfo) CSB-YP482093BQP
CSB-EP482093BQP
CSB-BP482093BQP
CSB-MP482093BQP
CSB-EP482093BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chaperone protein DnaK (dnaK), partial CSB-YP482094BQP
CSB-EP482094BQP
CSB-BP482094BQP
CSB-MP482094BQP
CSB-EP482094BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S20 (rpsT) CSB-YP482095BQP
CSB-EP482095BQP
CSB-BP482095BQP
CSB-MP482095BQP
CSB-EP482095BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0297 protein BCG9842_B0729 (BCG9842_B0729) CSB-YP482096BQP
CSB-EP482096BQP
CSB-BP482096BQP
CSB-MP482096BQP
CSB-EP482096BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 50S ribosomal protein L32 (rpmF) CSB-YP482097BUG
CSB-EP482097BUG
CSB-BP482097BUG
CSB-MP482097BUG
CSB-EP482097BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP482098BUG
CSB-EP482098BUG
CSB-BP482098BUG
CSB-MP482098BUG
CSB-EP482098BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP482099BUG
CSB-EP482099BUG
CSB-BP482099BUG
CSB-MP482099BUG
CSB-EP482099BUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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