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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human SH2 domain-containing protein 1A (SH2D1A) CSB-YP021209HU
CSB-EP021209HU
CSB-BP021209HU
CSB-MP021209HU
CSB-EP021209HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Oligophrenin-1 (OPHN1), partial CSB-YP016348HU
CSB-EP016348HU
CSB-BP016348HU
CSB-MP016348HU
CSB-EP016348HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Checkpoint protein HUS1 (HUS1) CSB-YP010909HU
CSB-BP010909HU
CSB-MP010909HU
CSB-EP010909HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ribonuclease H1 (RNASEH1) CSB-YP019801HU
CSB-BP019801HU
CSB-MP019801HU
CSB-EP019801HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Prepronociceptin (PNOC) CSB-YP018278BO
CSB-EP018278BO
CSB-BP018278BO
CSB-MP018278BO
CSB-EP018278BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Keratocan (KERA) CSB-YP012149BO
CSB-EP012149BO
CSB-BP012149BO
CSB-MP012149BO
CSB-EP012149BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Neuropeptide Y receptor type 5 (NPY5R), partial CSB-YP016037DO1
CSB-EP016037DO1
CSB-BP016037DO1
CSB-MP016037DO1
CSB-EP016037DO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cat Cholinesterase (BCHE), partial CSB-YP002604CA
CSB-EP002604CA
CSB-BP002604CA
CSB-MP002604CA
CSB-EP002604CA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Solute carrier family 2, facilitated glucose transporter member 2 (SLC2A2), partial CSB-YP021552PI1
CSB-EP021552PI1
CSB-BP021552PI1
CSB-MP021552PI1
CSB-EP021552PI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Antigen-presenting glycoprotein CD1d (CD1D), partial CSB-YP004892SH1
CSB-EP004892SH1
CSB-BP004892SH1
CSB-MP004892SH1
CSB-EP004892SH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse ATP-dependent RNA helicase A (Dhx9), partial CSB-YP006886MO
CSB-EP006886MO
CSB-BP006886MO
CSB-MP006886MO
CSB-EP006886MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calcium/calmodulin-dependent protein kinase type 1B (Pnck) CSB-YP018259RA
CSB-EP018259RA
CSB-BP018259RA
CSB-MP018259RA
CSB-EP018259RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ficolin-1 (Fcn1) CSB-YP008550MO
CSB-EP008550MO
CSB-BP008550MO
CSB-MP008550MO
CSB-EP008550MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit gamma (Pik3c2g), partial CSB-YP017996RA
CSB-EP017996RA
CSB-BP017996RA
CSB-MP017996RA
CSB-EP017996RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Prolyl endopeptidase (Prep) CSB-YP018664RA
CSB-EP018664RA
CSB-BP018664RA
CSB-MP018664RA
CSB-EP018664RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig 5-hydroxytryptamine receptor 3A (HTR3A), partial CSB-YP010890GU1
CSB-EP010890GU1
CSB-BP010890GU1
CSB-MP010890GU1
CSB-EP010890GU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Amyloid beta A4 precursor protein-binding family A member 3 (Apba3) CSB-YP001889RA
CSB-EP001889RA
CSB-BP001889RA
CSB-MP001889RA
CSB-EP001889RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat E3 SUMO-protein ligase PIAS3 (Pias3) CSB-YP017959RA
CSB-EP017959RA
CSB-BP017959RA
CSB-MP017959RA
CSB-EP017959RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat WAP four-disulfide core domain protein 1 (Wfdc1) CSB-YP026086RA
CSB-EP026086RA
CSB-BP026086RA
CSB-MP026086RA
CSB-EP026086RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Persephin (Pspn) CSB-YP018940MO
CSB-EP018940MO
CSB-BP018940MO
CSB-MP018940MO
CSB-EP018940MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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