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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Choline dehydrogenase (betA), partial CSB-YP005335SUI
CSB-EP005335SUI
CSB-BP005335SUI
CSB-MP005335SUI
CSB-EP005335SUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Adenine phosphoribosyltransferase (apt2) CSB-YP001954MNS
CSB-EP001954MNS
CSB-BP001954MNS
CSB-MP001954MNS
CSB-EP001954MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus aeolicus Argininosuccinate lyase (argH) CSB-YP002213MNO
CSB-EP002213MNO
CSB-BP002213MNO
CSB-MP002213MNO
CSB-EP002213MNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa CTP synthase (pyrG), partial CSB-YP006176PZL
CSB-EP006176PZL
CSB-BP006176PZL
CSB-MP006176PZL
CSB-EP006176PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Uracil-DNA glycosylase (ung) CSB-YP025641AUG
CSB-EP025641AUG
CSB-BP025641AUG
CSB-MP025641AUG
CSB-EP025641AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica GTP cyclohydrolase 1 (folE) CSB-YP009317STM
CSB-EP009317STM
CSB-BP009317STM
CSB-MP009317STM
CSB-EP009317STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Cytidine deaminase (cdd) CSB-YP004976STM
CSB-EP004976STM
CSB-BP004976STM
CSB-MP004976STM
CSB-EP004976STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Adenine phosphoribosyltransferase (apt) CSB-YP001954STM
CSB-EP001954STM
CSB-BP001954STM
CSB-MP001954STM
CSB-EP001954STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Thiopurine S-methyltransferase (tpm) CSB-YP024110STM
CSB-EP024110STM
CSB-BP024110STM
CSB-MP024110STM
CSB-EP024110STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Uroporphyrinogen decarboxylase (hemE) CSB-YP025678STM
CSB-EP025678STM
CSB-BP025678STM
CSB-MP025678STM
CSB-EP025678STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Histidine ammonia-lyase (hutH), partial CSB-YP010123ODH
CSB-EP010123ODH
CSB-BP010123ODH
CSB-MP010123ODH
CSB-EP010123ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Adenine phosphoribosyltransferase (apt) CSB-YP001954ODH
CSB-EP001954ODH
CSB-BP001954ODH
CSB-MP001954ODH
CSB-EP001954ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Adenine phosphoribosyltransferase (apt) CSB-YP001954NFA
CSB-EP001954NFA
CSB-BP001954NFA
CSB-MP001954NFA
CSB-EP001954NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Histidine ammonia-lyase (hutH), partial CSB-YP010123FLG
CSB-EP010123FLG
CSB-BP010123FLG
CSB-MP010123FLG
CSB-EP010123FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulata Adenylate kinase 1 (ADK1) CSB-YP001508AUN
CSB-EP001508AUN
CSB-BP001508AUN
CSB-MP001508AUN
CSB-EP001508AUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. CTP synthase (pyrG), partial CSB-YP006176JAQ
CSB-EP006176JAQ
CSB-BP006176JAQ
CSB-MP006176JAQ
CSB-EP006176JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Choline dehydrogenase (betA), partial CSB-YP005335KAX
CSB-EP005335KAX
CSB-BP005335KAX
CSB-MP005335KAX
CSB-EP005335KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Adenosine deaminase (add) CSB-YP001268KAX
CSB-EP001268KAX
CSB-BP001268KAX
CSB-MP001268KAX
CSB-EP001268KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae Aminomethyltransferase (gcvT) CSB-YP001684KAX
CSB-EP001684KAX
CSB-BP001684KAX
CSB-MP001684KAX
CSB-EP001684KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Argininosuccinate lyase (argH) CSB-YP002213MNS
CSB-EP002213MNS
CSB-BP002213MNS
CSB-MP002213MNS
CSB-EP002213MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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