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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Pseudomonas aeruginosa Thiopurine S-methyltransferase (tpm) CSB-YP024110PZL
CSB-EP024110PZL
CSB-BP024110PZL
CSB-MP024110PZL
CSB-EP024110PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Choline dehydrogenase (betA), partial CSB-YP005335PZL
CSB-EP005335PZL
CSB-BP005335PZL
CSB-MP005335PZL
CSB-EP005335PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Argininosuccinate lyase (argH) CSB-YP002213MNR
CSB-EP002213MNR
CSB-BP002213MNR
CSB-MP002213MNR
CSB-EP002213MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ornithine carbamoyltransferase (argF) CSB-YP017270MNR
CSB-EP017270MNR
CSB-BP017270MNR
CSB-MP017270MNR
CSB-EP017270MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica CTP synthase (pyrG), partial CSB-YP006176STM
CSB-EP006176STM
CSB-BP006176STM
CSB-MP006176STM
CSB-EP006176STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Argininosuccinate lyase (argH) CSB-YP002213ODH
CSB-EP002213ODH
CSB-BP002213ODH
CSB-MP002213ODH
CSB-EP002213ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Folylpolyglutamate synthase (MET7), partial CSB-YP008851STA
CSB-EP008851STA
CSB-BP008851STA
CSB-MP008851STA
CSB-EP008851STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial intermediate peptidase (OCT1), partial CSB-YP013835STA
CSB-EP013835STA
CSB-BP013835STA
CSB-MP013835STA
CSB-EP013835STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Ornithine carbamoyltransferase (argI) CSB-YP017270YAN
CSB-EP017270YAN
CSB-BP017270YAN
CSB-MP017270YAN
CSB-EP017270YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTP cyclohydrolase 1 (folE) CSB-YP009317CWU
CSB-EP009317CWU
CSB-BP009317CWU
CSB-MP009317CWU
CSB-EP009317CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Argininosuccinate lyase (argH) CSB-YP002213CWU
CSB-EP002213CWU
CSB-BP002213CWU
CSB-MP002213CWU
CSB-EP002213CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Uroporphyrinogen decarboxylase (hemE) CSB-YP025678BOK
CSB-EP025678BOK
CSB-BP025678BOK
CSB-MP025678BOK
CSB-EP025678BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus GTP cyclohydrolase 1 (folE) CSB-YP009317CWB
CSB-EP009317CWB
CSB-BP009317CWB
CSB-MP009317CWB
CSB-EP009317CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Ornithine carbamoyltransferase (arcB) CSB-YP017270CWB
CSB-EP017270CWB
CSB-BP017270CWB
CSB-MP017270CWB
CSB-EP017270CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Uroporphyrinogen decarboxylase (hemE) CSB-YP025678CWB
CSB-EP025678CWB
CSB-BP025678CWB
CSB-MP025678CWB
CSB-EP025678CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Adenine phosphoribosyltransferase (apt) CSB-YP001954CWE
CSB-EP001954CWE
CSB-BP001954CWE
CSB-MP001954CWE
CSB-EP001954CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis Argininosuccinate lyase (argH) CSB-YP002213CWD
CSB-EP002213CWD
CSB-BP002213CWD
CSB-MP002213CWD
CSB-EP002213CWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Boiga irregularis Glutaminyl-peptide cyclotransferase (QPCT) CSB-YP019135BPA
CSB-EP019135BPA
CSB-BP019135BPA
CSB-MP019135BPA
CSB-EP019135BPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Uracil-DNA glycosylase (ung) CSB-YP025641VEA
CSB-EP025641VEA
CSB-BP025641VEA
CSB-MP025641VEA
CSB-EP025641VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Glutaminase (glsA) CSB-YP009528VEA
CSB-EP009528VEA
CSB-BP009528VEA
CSB-MP009528VEA
CSB-EP009528VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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