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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica Thymidine phosphorylase (deoA) CSB-YP025392STN
CSB-EP025392STN
CSB-BP025392STN
CSB-MP025392STN
CSB-EP025392STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Aminomethyltransferase (gcvT) CSB-YP001684STN
CSB-EP001684STN
CSB-BP001684STN
CSB-MP001684STN
CSB-EP001684STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C Adenine phosphoribosyltransferase (apt) CSB-YP001954NEW
CSB-EP001954NEW
CSB-BP001954NEW
CSB-MP001954NEW
CSB-EP001954NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup C Uroporphyrinogen decarboxylase (hemE) CSB-YP025678NEW
CSB-EP025678NEW
CSB-BP025678NEW
CSB-MP025678NEW
CSB-EP025678NEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella canis Histidine ammonia-lyase (hutH), partial CSB-YP010123BPH
CSB-EP010123BPH
CSB-BP010123BPH
CSB-MP010123BPH
CSB-EP010123BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Uroporphyrinogen decarboxylase (hemE) CSB-YP025678STE
CSB-EP025678STE
CSB-BP025678STE
CSB-MP025678STE
CSB-EP025678STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Cytidine deaminase (cdd) CSB-YP004976STE
CSB-EP004976STE
CSB-BP004976STE
CSB-MP004976STE
CSB-EP004976STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Exoribonuclease 2 (rnb), partial CSB-YP007787STE
CSB-EP007787STE
CSB-BP007787STE
CSB-MP007787STE
CSB-EP007787STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Histidine ammonia-lyase (hutH), partial CSB-YP010123STF
CSB-EP010123STF
CSB-BP010123STF
CSB-MP010123STF
CSB-EP010123STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Exoribonuclease 2 (rnb), partial CSB-YP007787STF
CSB-EP007787STF
CSB-BP007787STF
CSB-MP007787STF
CSB-EP007787STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii CTP synthase (pyrG), partial CSB-YP006176CXH
CSB-EP006176CXH
CSB-BP006176CXH
CSB-MP006176CXH
CSB-EP006176CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Glutaminase (glsA) CSB-YP009528BON
CSB-EP009528BON
CSB-BP009528BON
CSB-MP009528BON
CSB-EP009528BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Adenine phosphoribosyltransferase (apt) CSB-YP001954DSX
CSB-EP001954DSX
CSB-BP001954DSX
CSB-MP001954DSX
CSB-EP001954DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio anubis Cingulin (CGN), partial CSB-YP005302ERI
CSB-EP005302ERI
CSB-BP005302ERI
CSB-MP005302ERI
CSB-EP005302ERI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus CTP synthase (pyrG), partial CSB-YP006176PZC
CSB-EP006176PZC
CSB-BP006176PZC
CSB-MP006176PZC
CSB-EP006176PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans CTP synthase (pyrG), partial CSB-YP006176DHV
CSB-EP006176DHV
CSB-BP006176DHV
CSB-MP006176DHV
CSB-EP006176DHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorangium cellulosum Ornithine carbamoyltransferase (argF) CSB-YP017270SUD
CSB-EP017270SUD
CSB-BP017270SUD
CSB-MP017270SUD
CSB-EP017270SUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus Uroporphyrinogen decarboxylase (hemE) CSB-YP025678GEN
CSB-EP025678GEN
CSB-BP025678GEN
CSB-MP025678GEN
CSB-EP025678GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter diazotrophicus CTP synthase (pyrG), partial CSB-YP006176GEN
CSB-EP006176GEN
CSB-BP006176GEN
CSB-MP006176GEN
CSB-EP006176GEN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Uroporphyrinogen decarboxylase (hemE) CSB-YP025678BPB
CSB-EP025678BPB
CSB-BP025678BPB
CSB-MP025678BPB
CSB-EP025678BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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