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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella arizonae Thymidine phosphorylase (deoA) CSB-YP025392STE
CSB-EP025392STE
CSB-BP025392STE
CSB-MP025392STE
CSB-EP025392STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B 6-phosphogluconolactonase (pgl) CSB-YP017861STF
CSB-EP017861STF
CSB-BP017861STF
CSB-MP017861STF
CSB-EP017861STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Adenine phosphoribosyltransferase (apt) CSB-YP001954STF
CSB-EP001954STF
CSB-BP001954STF
CSB-MP001954STF
CSB-EP001954STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Spermidine synthase (speE) CSB-YP022668STF
CSB-EP022668STF
CSB-BP022668STF
CSB-MP022668STF
CSB-EP022668STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Adenine phosphoribosyltransferase (apt) CSB-YP001954ATX
CSB-EP001954ATX
CSB-BP001954ATX
CSB-MP001954ATX
CSB-EP001954ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Ancient ubiquitous protein 1 (aup1), partial CSB-YP002453XBF1
CSB-EP002453XBF1
CSB-BP002453XBF1
CSB-MP002453XBF1
CSB-EP002453XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Aminomethyltransferase (gcvT) CSB-YP001684BON
CSB-EP001684BON
CSB-BP001684BON
CSB-MP001684BON
CSB-EP001684BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Uroporphyrinogen decarboxylase (hemE) CSB-YP025678BON
CSB-EP025678BON
CSB-BP025678BON
CSB-MP025678BON
CSB-EP025678BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Uroporphyrinogen decarboxylase (hemE) CSB-YP025678DSX
CSB-EP025678DSX
CSB-BP025678DSX
CSB-MP025678DSX
CSB-EP025678DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus CTP synthase (pyrG), partial CSB-YP006176DSX
CSB-EP006176DSX
CSB-BP006176DSX
CSB-MP006176DSX
CSB-EP006176DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Argininosuccinate lyase (argH) CSB-YP002213DSX
CSB-EP002213DSX
CSB-BP002213DSX
CSB-MP002213DSX
CSB-EP002213DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Histidine ammonia-lyase (hutH), partial CSB-YP010123BWU
CSB-EP010123BWU
CSB-BP010123BWU
CSB-MP010123BWU
CSB-EP010123BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Tryptophan 2,3-dioxygenase (kynA) CSB-YP023351BPS
CSB-EP023351BPS
CSB-BP023351BPS
CSB-MP023351BPS
CSB-EP023351BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Adenine phosphoribosyltransferase (apt) CSB-YP001954PYI
CSB-EP001954PYI
CSB-BP001954PYI
CSB-MP001954PYI
CSB-EP001954PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii Adenine phosphoribosyltransferase (apt) CSB-YP001954BPB
CSB-EP001954BPB
CSB-BP001954BPB
CSB-MP001954BPB
CSB-EP001954BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella petrii CTP synthase (pyrG), partial CSB-YP006176BPB
CSB-EP006176BPB
CSB-BP006176BPB
CSB-MP006176BPB
CSB-EP006176BPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Uracil-DNA glycosylase (ung) CSB-YP025641DXO
CSB-EP025641DXO
CSB-BP025641DXO
CSB-MP025641DXO
CSB-EP025641DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Uroporphyrinogen decarboxylase (hemE) CSB-YP025678DXO
CSB-EP025678DXO
CSB-BP025678DXO
CSB-MP025678DXO
CSB-EP025678DXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium phytofermentans Argininosuccinate lyase (argH) CSB-YP002213CWZ
CSB-EP002213CWZ
CSB-BP002213CWZ
CSB-MP002213CWZ
CSB-EP002213CWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glutaminase (glsA) CSB-YP009528STN
CSB-EP009528STN
CSB-BP009528STN
CSB-MP009528STN
CSB-EP009528STN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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