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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rat B1 bradykinin receptor (Bdkrb1), partial CSB-YP002651RA1
CSB-EP002651RA1
CSB-BP002651RA1
CSB-MP002651RA1
CSB-EP002651RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Casein kinase I isoform alpha (Csnk1a1) CSB-YP006065RA
CSB-EP006065RA
CSB-BP006065RA
CSB-MP006065RA
CSB-EP006065RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemur catta Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073LOK1
CSB-EP015073LOK1
CSB-BP015073LOK1
CSB-MP015073LOK1
CSB-EP015073LOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus vulcanus Cytochrome c oxidase subunit 2 (ctaC), partial CSB-YP015073TNI1
CSB-EP015073TNI1
CSB-BP015073TNI1
CSB-MP015073TNI1
CSB-EP015073TNI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917SKY
CSB-EP022917SKY
CSB-BP022917SKY
CSB-MP022917SKY
CSB-EP022917SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582SKY
CSB-EP009582SKY
CSB-BP009582SKY
CSB-MP009582SKY
CSB-EP009582SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Triosephosphate isomerase (tpiA) CSB-YP024102SKY
CSB-EP024102SKY
CSB-BP024102SKY
CSB-MP024102SKY
CSB-EP024102SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 6-phosphofructokinase (pfkA) CSB-YP017822SKY
CSB-EP017822SKY
CSB-BP017822SKY
CSB-MP017822SKY
CSB-EP017822SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Elongation factor Ts (tsf) CSB-YP025124SKY
CSB-EP025124SKY
CSB-BP025124SKY
CSB-MP025124SKY
CSB-EP025124SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus edulis Cytochrome c oxidase subunit 2 (COII), partial CSB-YP015073MWU1
CSB-EP015073MWU1
CSB-BP015073MWU1
CSB-MP015073MWU1
CSB-EP015073MWU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans DNA topoisomerase 1 (TOP1), partial CSB-YP024056CZF
CSB-EP024056CZF
CSB-BP024056CZF
CSB-MP024056CZF
CSB-EP024056CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus flavus Uricase (uaZ) CSB-YP025648AVD
CSB-EP025648AVD
CSB-BP025648AVD
CSB-MP025648AVD
CSB-EP025648AVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis FACT complex subunit SPT16 (SPT16), partial CSB-YP022955KBK
CSB-EP022955KBK
CSB-BP022955KBK
CSB-MP022955KBK
CSB-EP022955KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepilemur sahamalazensis NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080LED1
CSB-EP015080LED1
CSB-BP015080LED1
CSB-MP015080LED1
CSB-EP015080LED1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase I subunit RPA49 (RPA49) CSB-YP018326SVG
CSB-EP018326SVG
CSB-BP018326SVG
CSB-MP018326SVG
CSB-EP018326SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tropomyosin alpha-1 chain (tpm1) CSB-YP024104XBE
CSB-EP024104XBE
CSB-BP024104XBE
CSB-MP024104XBE
CSB-EP024104XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthopagrus butcheri Somatotropin (gh) CSB-YP009407AAL
CSB-EP009407AAL
CSB-BP009407AAL
CSB-MP009407AAL
CSB-EP009407AAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichophyton rubrum NADH-ubiquinone oxidoreductase chain 5 (ND5), partial CSB-YP015081TQA
CSB-EP015081TQA
CSB-BP015081TQA
CSB-MP015081TQA
CSB-EP015081TQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A synthetase (facA), partial CSB-YP001203PIL
CSB-EP001203PIL
CSB-BP001203PIL
CSB-MP001203PIL
CSB-EP001203PIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein Shroom1 (shroom1), partial CSB-YP021281XBE
CSB-EP021281XBE
CSB-BP021281XBE
CSB-MP021281XBE
CSB-EP021281XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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