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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 6-phosphofructokinase (pfkA), partial CSB-YP017822DOZ
CSB-EP017822DOZ
CSB-BP017822DOZ
CSB-MP017822DOZ
CSB-EP017822DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces pastorianus Protein RER1 (RER1), partial CSB-YP019567SAJ1
CSB-EP019567SAJ1
CSB-BP019567SAJ1
CSB-MP019567SAJ1
CSB-EP019567SAJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription initiation factor IIE subunit beta (tfa2) CSB-YP010003SXV
CSB-EP010003SXV
CSB-BP010003SXV
CSB-MP010003SXV
CSB-EP010003SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Eukaryotic peptide chain release factor subunit 1 (sup45) CSB-YP007840SXV
CSB-EP007840SXV
CSB-BP007840SXV
CSB-MP007840SXV
CSB-EP007840SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Membrane cofactor protein (CD46), partial CSB-YP004939DSU1
CSB-EP004939DSU1
CSB-BP004939DSU1
CSB-MP004939DSU1
CSB-EP004939DSU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla fMet-Leu-Phe receptor (FPR1), partial CSB-YP008854GGZ1
CSB-EP008854GGZ1
CSB-BP008854GGZ1
CSB-MP008854GGZ1
CSB-EP008854GGZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fuscata fuscata T-cell surface glycoprotein CD4 (CD4), partial CSB-YP004935MMX1
CSB-EP004935MMX1
CSB-BP004935MMX1
CSB-MP004935MMX1
CSB-EP004935MMX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus N-formyl peptide receptor 2 (FPR2), partial CSB-YP008855EXP1
CSB-EP008855EXP1
CSB-BP008855EXP1
CSB-MP008855EXP1
CSB-EP008855EXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes N-formyl peptide receptor 3 (FPR3), partial CSB-YP008856EQV1
CSB-EP008856EQV1
CSB-BP008856EQV1
CSB-MP008856EQV1
CSB-EP008856EQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Indian hedgehog protein (ihh) CSB-YP011569DQG
CSB-EP011569DQG
CSB-BP011569DQG
CSB-MP011569DQG
CSB-EP011569DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio GTPase NRas (nras) CSB-YP016070DIL
CSB-EP016070DIL
CSB-BP016070DIL
CSB-MP016070DIL
CSB-EP016070DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes 5-hydroxytryptamine receptor 1D (htr1d), partial CSB-YP010883TKY1
CSB-EP010883TKY1
CSB-BP010883TKY1
CSB-MP010883TKY1
CSB-EP010883TKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcebus murinus Presenilin-1 (PSEN1), partial CSB-YP018846MFU
CSB-EP018846MFU
CSB-BP018846MFU
CSB-MP018846MFU
CSB-EP018846MFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sonic hedgehog protein (shh) CSB-YP021266FGX
CSB-EP021266FGX
CSB-BP021266FGX
CSB-MP021266FGX
CSB-EP021266FGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Puntius tetrazona Sonic hedgehog protein (shh) CSB-YP021266PXC
CSB-EP021266PXC
CSB-BP021266PXC
CSB-MP021266PXC
CSB-EP021266PXC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tanichthys albonubes Sonic hedgehog protein (shh) CSB-YP021266TAR
CSB-EP021266TAR
CSB-BP021266TAR
CSB-MP021266TAR
CSB-EP021266TAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nocturnin (ccrn4l) CSB-YP004853XBE
CSB-EP004853XBE
CSB-BP004853XBE
CSB-MP004853XBE
CSB-EP004853XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phascolarctos cinereus Sperm protamine P1 (PRM1) CSB-YP018728EUM
CSB-EP018728EUM
CSB-BP018728EUM
CSB-MP018728EUM
CSB-EP018728EUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Malate dehydrogenase (mdh) CSB-YP013623DST
CSB-EP013623DST
CSB-BP013623DST
CSB-MP013623DST
CSB-EP013623DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fuscata fuscata Parvalbumin alpha (PVALB) CSB-YP019092MMX
CSB-EP019092MMX
CSB-BP019092MMX
CSB-MP019092MMX
CSB-EP019092MMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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