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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mitochondrial import inner membrane translocase subunit TIM14 (dnj-21), partial CSB-YP007026CXY1
CSB-EP007026CXY1
CSB-BP007026CXY1
CSB-MP007026CXY1
CSB-EP007026CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Histone H4 CSB-YP010429DNK
CSB-EP010429DNK
CSB-BP010429DNK
CSB-MP010429DNK
CSB-EP010429DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078DOA1
CSB-EP015078DOA1
CSB-BP015078DOA1
CSB-MP015078DOA1
CSB-EP015078DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachesis muta muta NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079LMC1
CSB-EP015079LMC1
CSB-BP015079LMC1
CSB-MP015079LMC1
CSB-EP015079LMC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macropus robustus Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073MAK1
CSB-EP015073MAK1
CSB-BP015073MAK1
CSB-MP015073MAK1
CSB-EP015073MAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073PYX1
CSB-EP015073PYX1
CSB-BP015073PYX1
CSB-MP015073PYX1
CSB-EP015073PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii NADH-ubiquinone oxidoreductase chain 5 (MT-ND5), partial CSB-YP015081PYX
CSB-EP015081PYX
CSB-BP015081PYX
CSB-MP015081PYX
CSB-EP015081PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paralichthys olivaceus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078ERT1
CSB-EP015078ERT1
CSB-BP015078ERT1
CSB-MP015078ERT1
CSB-EP015078ERT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachesis muta muta Cytochrome b (MT-CYB), partial CSB-YP015075LMC1
CSB-EP015075LMC1
CSB-BP015075LMC1
CSB-MP015075LMC1
CSB-EP015075LMC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome c oxidase subunit 2 (COX2), partial CSB-YP015073DOA1
CSB-EP015073DOA1
CSB-BP015073DOA1
CSB-MP015073DOA1
CSB-EP015073DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 (NAD7) CSB-YP015661DOA
CSB-EP015661DOA
CSB-BP015661DOA
CSB-MP015661DOA
CSB-EP015661DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NADH-ubiquinone oxidoreductase chain 4 (ND4), partial CSB-YP015079DOA
CSB-EP015079DOA
CSB-BP015079DOA
CSB-MP015079DOA
CSB-EP015079DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 40S ribosomal protein S14 (RPS14) CSB-YP020371NHE
CSB-EP020371NHE
CSB-BP020371NHE
CSB-MP020371NHE
CSB-EP020371NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera berteriana NADH-ubiquinone oxidoreductase chain 2 (ND2), partial CSB-YP015077OAR
CSB-EP015077OAR
CSB-BP015077OAR
CSB-MP015077OAR
CSB-EP015077OAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA repair protein RAD51 homolog 1 (RAD51) CSB-YP019263DOA
CSB-EP019263DOA
CSB-BP019263DOA
CSB-MP019263DOA
CSB-EP019263DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces cinnamoneus Elongation factor Tu (tuf) CSB-YP025342SNY
CSB-EP025342SNY
CSB-BP025342SNY
CSB-MP025342SNY
CSB-EP025342SNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit alpha (atpA), partial CSB-YP002344FMR
CSB-EP002344FMR
CSB-BP002344FMR
CSB-MP002344FMR
CSB-EP002344FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Multidrug resistance protein 3 (bmr3), partial CSB-YP001050BRJ
CSB-EP001050BRJ
CSB-BP001050BRJ
CSB-MP001050BRJ
CSB-EP001050BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus DNA polymerase delta catalytic subunit (POLD1), partial CSB-YP018303MRG
CSB-EP018303MRG
CSB-BP018303MRG
CSB-MP018303MRG
CSB-EP018303MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Interleukin-15 (Il15) CSB-YP011593RA
CSB-EP011593RA
CSB-BP011593RA
CSB-MP011593RA
CSB-EP011593RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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