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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cytochrome c oxidase subunit 1 (COI), partial CSB-YP015072TLT
CSB-EP015072TLT
CSB-BP015072TLT
CSB-MP015072TLT
CSB-EP015072TLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Squalus acanthias Relaxin CSB-YP019749FKW
CSB-EP019749FKW
CSB-BP019749FKW
CSB-MP019749FKW
CSB-EP019749FKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Metallothionein-1A CSB-YP015107RB
CSB-EP015107RB
CSB-BP015107RB
CSB-MP015107RB
CSB-EP015107RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Asterina pectinifera NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078DPG1
CSB-EP015078DPG1
CSB-BP015078DPG1
CSB-MP015078DPG1
CSB-EP015078DPG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA ligase 1 (cdc17), partial CSB-YP012930SXV
CSB-EP012930SXV
CSB-BP012930SXV
CSB-MP012930SXV
CSB-EP012930SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio cynocephalus Alpha-lactalbumin (LALBA) CSB-YP012724PBR
CSB-EP012724PBR
CSB-BP012724PBR
CSB-MP012724PBR
CSB-EP012724PBR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia salina Elongation factor 1-gamma CSB-YP007433AOE
CSB-EP007433AOE
CSB-BP007433AOE
CSB-MP007433AOE
CSB-EP007433AOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Liver carboxylesterase 1, partial CSB-YP005258RB
CSB-EP005258RB
CSB-BP005258RB
CSB-MP005258RB
CSB-EP005258RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Eukaryotic peptide chain release factor subunit 1 (SUP45) CSB-YP007840SVG
CSB-EP007840SVG
CSB-BP007840SVG
CSB-MP007840SVG
CSB-EP007840SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. ATP synthase subunit alpha (atpA), partial CSB-YP002344NHR
CSB-EP002344NHR
CSB-BP002344NHR
CSB-MP002344NHR
CSB-EP002344NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Tubulin beta-1 chain (TUBB1) CSB-YP025319DOA
CSB-EP025319DOA
CSB-BP025319DOA
CSB-MP025319DOA
CSB-EP025319DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin beta chain (TUBB) CSB-YP025318EHU
CSB-EP025318EHU
CSB-BP025318EHU
CSB-MP025318EHU
CSB-EP025318EHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaseolus vulgaris NADP-dependent malic enzyme (ME1), partial CSB-YP013634EUN
CSB-EP013634EUN
CSB-BP013634EUN
CSB-MP013634EUN
CSB-EP013634EUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracentrotus lividus Cytochrome c oxidase subunit 3 (COIII), partial CSB-YP015074ERM1
CSB-EP015074ERM1
CSB-BP015074ERM1
CSB-MP015074ERM1
CSB-EP015074ERM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Translation initiation factor eIF-2B subunit delta (GCD2), partial CSB-YP007517SVG
CSB-EP007517SVG
CSB-BP007517SVG
CSB-MP007517SVG
CSB-EP007517SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracentrotus lividus NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078ERM1
CSB-EP015078ERM1
CSB-BP015078ERM1
CSB-MP015078ERM1
CSB-EP015078ERM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracentrotus lividus NADH-ubiquinone oxidoreductase chain 6 (ND6), partial CSB-YP015082ERM1
CSB-EP015082ERM1
CSB-BP015082ERM1
CSB-MP015082ERM1
CSB-EP015082ERM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sarcophaga peregrina Cytochrome c CSB-YP006328SCK
CSB-EP006328SCK
CSB-BP006328SCK
CSB-MP006328SCK
CSB-EP006328SCK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Neurofilament medium polypeptide (Nefm), partial CSB-YP015691RA
CSB-EP015691RA
CSB-BP015691RA
CSB-MP015691RA
CSB-EP015691RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit a (atpB), partial CSB-YP015070VEU1
CSB-EP015070VEU1
CSB-BP015070VEU1
CSB-MP015070VEU1
CSB-EP015070VEU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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