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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pongo abelii NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (NDUFA8) CSB-YP015633PYX
CSB-EP015633PYX
CSB-BP015633PYX
CSB-MP015633PYX
CSB-EP015633PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (NDUFB10) CSB-YP015642PYX
CSB-EP015642PYX
CSB-BP015642PYX
CSB-MP015642PYX
CSB-EP015642PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033DSB
CSB-EP018033DSB
CSB-BP018033DSB
CSB-MP018033DSB
CSB-EP018033DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Chromosome transmission fidelity protein 8 homolog isoform 2 (CHTF8) CSB-YP005419HU
CSB-EP005419HU
CSB-BP005419HU
CSB-MP005419HU
CSB-EP005419HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Histone lysine demethylase PHF8 (phf8), partial CSB-YP017919DIL
CSB-EP017919DIL
CSB-BP017919DIL
CSB-MP017919DIL
CSB-EP017919DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops marajoensis L-amino-acid oxidase CSB-YP011660BUY
CSB-EP011660BUY
CSB-BP011660BUY
CSB-MP011660BUY
CSB-EP011660BUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Solute carrier family 35 member E2B (SLC35E2B), partial CSB-YP021598HU
CSB-EP021598HU
CSB-BP021598HU
CSB-MP021598HU
CSB-EP021598HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582BUD
CSB-EP009582BUD
CSB-BP009582BUD
CSB-MP009582BUD
CSB-EP009582BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Acyl-protein thioesterase 1 (CNBF2260) CSB-YP013268CTK
CSB-EP013268CTK
CSB-BP013268CTK
CSB-MP013268CTK
CSB-EP013268CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy protein 5 (ATG5) CSB-YP002293CTK
CSB-EP002293CTK
CSB-BP002293CTK
CSB-MP002293CTK
CSB-EP002293CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial intermembrane space import and assembly protein 40 (MIA40), partial CSB-YP005319CTL1
CSB-EP005319CTL1
CSB-BP005319CTL1
CSB-MP005319CTL1
CSB-EP005319CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Conserved oligomeric Golgi complex subunit 6 (COG6), partial CSB-YP005710CTK
CSB-EP005710CTK
CSB-BP005710CTK
CSB-MP005710CTK
CSB-EP005710CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Cytochrome c oxidase assembly protein COX19 (COX19) CSB-YP005831CTL
CSB-EP005831CTL
CSB-BP005831CTL
CSB-MP005831CTL
CSB-EP005831CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Eukaryotic translation initiation factor 3 subunit M (CNA04920) CSB-YP007542CTL
CSB-EP007542CTL
CSB-BP007542CTL
CSB-MP007542CTL
CSB-EP007542CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Diphthamide biosynthesis protein 2 (DPH2), partial CSB-YP007130CTL
CSB-EP007130CTL
CSB-BP007130CTL
CSB-MP007130CTL
CSB-EP007130CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Methylthioribose-1-phosphate isomerase (MRI1) CSB-YP014799CTK
CSB-EP014799CTK
CSB-BP014799CTK
CSB-MP014799CTK
CSB-EP014799CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Eukaryotic translation initiation factor 3 subunit C (NIP1), partial CSB-YP007531CTL
CSB-EP007531CTL
CSB-BP007531CTL
CSB-MP007531CTL
CSB-EP007531CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Eukaryotic translation initiation factor 3 subunit G (TIF35) CSB-YP007536CTK
CSB-EP007536CTK
CSB-BP007536CTK
CSB-MP007536CTK
CSB-EP007536CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Mitochondrial import inner membrane translocase subunit TIM50 (TIM50), partial CSB-YP023556CTL1
CSB-EP023556CTL1
CSB-BP023556CTL1
CSB-MP023556CTL1
CSB-EP023556CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D H/ACA ribonucleoprotein complex subunit 1 (GAR1) CSB-YP009257CTL
CSB-EP009257CTL
CSB-BP009257CTL
CSB-MP009257CTL
CSB-EP009257CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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