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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acanthophis antarcticus Phospholipase A2 CSB-YP018091AAO
CSB-EP018091AAO
CSB-BP018091AAO
CSB-MP018091AAO
CSB-EP018091AAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio hamadryas Protein disulfide-isomerase A3 (PDIA3) CSB-YP017720ERJ
CSB-EP017720ERJ
CSB-BP017720ERJ
CSB-MP017720ERJ
CSB-EP017720ERJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alligator mississippiensis Cytochrome c CSB-YP006328AZO
CSB-EP006328AZO
CSB-BP006328AZO
CSB-MP006328AZO
CSB-EP006328AZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b (petB), partial CSB-YP015075RLX
CSB-EP015075RLX
CSB-BP015075RLX
CSB-MP015075RLX
CSB-EP015075RLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Thioredoxin reductase (trxB) CSB-YP025388BXE
CSB-EP025388BXE
CSB-BP025388BXE
CSB-MP025388BXE
CSB-EP025388BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Interstitial collagenase (Mmp1), partial CSB-YP014656RA
CSB-EP014656RA
CSB-BP014656RA
CSB-MP014656RA
CSB-EP014656RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaline phosphatase CSB-YP001633EYG
CSB-EP001633EYG
CSB-BP001633EYG
CSB-MP001633EYG
CSB-EP001633EYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Tubulin alpha-1B chain CSB-YP025307BO
CSB-EP025307BO
CSB-BP025307BO
CSB-MP025307BO
CSB-EP025307BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Beta-defensin 2 (Defb2) CSB-YP006705MO
CSB-EP006705MO
CSB-BP006705MO
CSB-MP006705MO
CSB-EP006705MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cervus nippon nippon Cytochrome b (MT-CYB), partial CSB-YP015075CHF1
CSB-EP015075CHF1
CSB-BP015075CHF1
CSB-MP015075CHF1
CSB-EP015075CHF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cervus nippon aplodontus Cytochrome b (MT-CYB), partial CSB-YP015075CHD1
CSB-EP015075CHD1
CSB-BP015075CHD1
CSB-MP015075CHD1
CSB-EP015075CHD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrurus nigrocinctus Phospholipase A2 CSB-YP018091MGO
CSB-EP018091MGO
CSB-BP018091MGO
CSB-MP018091MGO
CSB-EP018091MGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Transcription elongation factor SPT4 (SPT4) CSB-YP022957KBK
CSB-EP022957KBK
CSB-BP022957KBK
CSB-MP022957KBK
CSB-EP022957KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops jararaca Phospholipase A2 CSB-YP018091BUW
CSB-EP018091BUW
CSB-BP018091BUW
CSB-MP018091BUW
CSB-EP018091BUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triosephosphate isomerase (tpiA) CSB-YP024102CMA
CSB-EP024102CMA
CSB-BP024102CMA
CSB-MP024102CMA
CSB-EP024102CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrovipera lebetina L-amino-acid oxidase CSB-YP011660MPJ
CSB-EP011660MPJ
CSB-BP011660MPJ
CSB-MP011660MPJ
CSB-EP011660MPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Cytochrome b (COB), partial CSB-YP015075KBK
CSB-EP015075KBK
CSB-BP015075KBK
CSB-MP015075KBK
CSB-EP015075KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Neutrophil defensin 4 CSB-YP006656MRG
CSB-EP006656MRG
CSB-BP006656MRG
CSB-MP006656MRG
CSB-EP006656MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Torpedo californica Vesicular acetylcholine transporter, partial CSB-YP021428TOT
CSB-EP021428TOT
CSB-BP021428TOT
CSB-MP021428TOT
CSB-EP021428TOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Nucleoside diphosphate kinase 3 CSB-YP015888FKI
CSB-EP015888FKI
CSB-BP015888FKI
CSB-MP015888FKI
CSB-EP015888FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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