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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Mitochondrial import inner membrane translocase subunit TIM44 (TIM44), partial CSB-YP023555SVG
CSB-EP023555SVG
CSB-BP023555SVG
CSB-MP023555SVG
CSB-EP023555SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Dual specificity mitogen-activated protein kinase kinase 1 (Map2k1) CSB-YP013409RA
CSB-EP013409RA
CSB-BP013409RA
CSB-MP013409RA
CSB-EP013409RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Selenoprotein N (sepn1), partial CSB-YP021017XBF1
CSB-EP021017XBF1
CSB-BP021017XBF1
CSB-MP021017XBF1
CSB-EP021017XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Triosephosphate isomerase (tpiA) CSB-YP024102SIU
CSB-EP024102SIU
CSB-BP024102SIU
CSB-MP024102SIU
CSB-EP024102SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Translation initiation factor IF-2 (infB), partial CSB-YP015164SIU
CSB-EP015164SIU
CSB-BP015164SIU
CSB-MP015164SIU
CSB-EP015164SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824SIU
CSB-EP005824SIU
CSB-BP005824SIU
CSB-MP005824SIU
CSB-EP005824SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delta-aminolevulinic acid dehydratase (hemB) CSB-YP001558MFG
CSB-EP001558MFG
CSB-BP001558MFG
CSB-MP001558MFG
CSB-EP001558MFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SIU
CSB-EP015150SIU
CSB-BP015150SIU
CSB-MP015150SIU
CSB-EP015150SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Cytochrome b (petB), partial CSB-YP015075RLE
CSB-EP015075RLE
CSB-BP015075RLE
CSB-MP015075RLE
CSB-EP015075RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae tRNA-splicing endonuclease subunit SEN54 (SEN54) CSB-YP025122SVG
CSB-EP025122SVG
CSB-BP025122SVG
CSB-MP025122SVG
CSB-EP025122SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146SIU
CSB-EP019146SIU
CSB-BP019146SIU
CSB-MP019146SIU
CSB-EP019146SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus L-threonine 3-dehydrogenase (tdh) CSB-YP023350SIU
CSB-EP023350SIU
CSB-BP023350SIU
CSB-MP023350SIU
CSB-EP023350SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus ATP synthase subunit b (atpF), partial CSB-YP002358SIU1
CSB-EP002358SIU1
CSB-BP002358SIU1
CSB-MP002358SIU1
CSB-EP002358SIU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Cardiolipin synthase (cls), partial CSB-YP005985PUC1
CSB-EP005985PUC1
CSB-BP005985PUC1
CSB-MP005985PUC1
CSB-EP005985PUC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717PUC
CSB-EP009717PUC
CSB-BP009717PUC
CSB-MP009717PUC
CSB-EP009717PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917PUC
CSB-EP022917PUC
CSB-BP022917PUC
CSB-MP022917PUC
CSB-EP022917PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Enolase-phosphatase E1 (mtnC) CSB-YP007674PUC
CSB-EP007674PUC
CSB-BP007674PUC
CSB-MP007674PUC
CSB-EP007674PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Thymidylate kinase (tmk) CSB-YP007226PUC
CSB-EP007226PUC
CSB-BP007226PUC
CSB-MP007226PUC
CSB-EP007226PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoserine aminotransferase (serC) CSB-YP018838PUC
CSB-EP018838PUC
CSB-BP018838PUC
CSB-MP018838PUC
CSB-EP018838PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Glucokinase (glk) CSB-YP009319PUC
CSB-EP009319PUC
CSB-BP009319PUC
CSB-MP009319PUC
CSB-EP009319PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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