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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas aeruginosa Argininosuccinate synthase (argG) CSB-YP002234PUC
CSB-EP002234PUC
CSB-BP002234PUC
CSB-MP002234PUC
CSB-EP002234PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Ribosome-recycling factor (frr) CSB-YP014941LBE
CSB-EP014941LBE
CSB-BP014941LBE
CSB-MP014941LBE
CSB-EP014941LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Glutamate receptor 1 (Glu-RI), partial CSB-YP009898DLU
CSB-EP009898DLU
CSB-BP009898DLU
CSB-MP009898DLU
CSB-EP009898DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytoplasmic polyadenylation element-binding protein 1 (cpb-1), partial CSB-YP005887CXY
CSB-EP005887CXY
CSB-BP005887CXY
CSB-MP005887CXY
CSB-EP005887CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida parapsilosis ATP synthase subunit a (ATP6), partial CSB-YP015070CZK1
CSB-EP015070CZK1
CSB-BP015070CZK1
CSB-MP015070CZK1
CSB-EP015070CZK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei ATP synthase subunit b (atpF), partial CSB-YP002358LAN1
CSB-EP002358LAN1
CSB-BP002358LAN1
CSB-MP002358LAN1
CSB-EP002358LAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Triosephosphate isomerase (tpiA) CSB-YP024102LAN
CSB-EP024102LAN
CSB-BP024102LAN
CSB-MP024102LAN
CSB-EP024102LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediococcus pentosaceus Serine hydroxymethyltransferase (glyA) CSB-YP021274PEE
CSB-EP021274PEE
CSB-BP021274PEE
CSB-MP021274PEE
CSB-EP021274PEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus thermophilus Galactokinase (galK) CSB-YP009198SNF
CSB-EP009198SNF
CSB-BP009198SNF
CSB-MP009198SNF
CSB-EP009198SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus thermophilus Peptide chain release factor 1 (prfA) CSB-YP015197SNF
CSB-EP015197SNF
CSB-BP015197SNF
CSB-MP015197SNF
CSB-EP015197SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus thermophilus Thymidylate kinase (tmk) CSB-YP007226SNF
CSB-EP007226SNF
CSB-BP007226SNF
CSB-MP007226SNF
CSB-EP007226SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Elongation factor Tu (tuf) CSB-YP025342LAL
CSB-EP025342LAL
CSB-BP025342LAL
CSB-MP025342LAL
CSB-EP025342LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Glucose-6-phosphate isomerase (pgi) CSB-YP009717LAL
CSB-EP009717LAL
CSB-BP009717LAL
CSB-MP009717LAL
CSB-EP009717LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Argininosuccinate synthase (argG) CSB-YP002234LFG
CSB-EP002234LFG
CSB-BP002234LFG
CSB-MP002234LFG
CSB-EP002234LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc mesenteroides subsp. mesenteroides Thymidylate kinase (tmk) CSB-YP007226LFG
CSB-EP007226LFG
CSB-BP007226LFG
CSB-MP007226LFG
CSB-EP007226LFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GPI mannosyltransferase 4 (SMP3), partial CSB-YP017989SVG
CSB-EP017989SVG
CSB-BP017989SVG
CSB-MP017989SVG
CSB-EP017989SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri tRNA dimethylallyltransferase (miaA) CSB-YP024529LAU
CSB-EP024529LAU
CSB-BP024529LAU
CSB-MP024529LAU
CSB-EP024529LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcription initiation factor TFIID subunit 11 (TAF11) CSB-YP023078SVG
CSB-EP023078SVG
CSB-BP023078SVG
CSB-MP023078SVG
CSB-EP023078SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus gasseri ATP synthase subunit alpha (atpA), partial CSB-YP002344LAU
CSB-EP002344LAU
CSB-BP002344LAU
CSB-MP002344LAU
CSB-EP002344LAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase III subunit RPC10 (RPC11) CSB-YP018352SVG
CSB-EP018352SVG
CSB-BP018352SVG
CSB-MP018352SVG
CSB-EP018352SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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