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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma genitalium Elongation factor Tu (tuf) CSB-YP025342MLN
CSB-EP025342MLN
CSB-BP025342MLN
CSB-MP025342MLN
CSB-EP025342MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Dolichol-phosphate mannosyltransferase (DPM1), partial CSB-YP007134SVG1
CSB-EP007134SVG1
CSB-BP007134SVG1
CSB-MP007134SVG1
CSB-EP007134SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis 60S ribosomal protein L4 (rpl4) CSB-YP020289XBF
CSB-EP020289XBF
CSB-BP020289XBF
CSB-MP020289XBF
CSB-EP020289XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L3 (RPL3) CSB-YP020224SVG
CSB-EP020224SVG
CSB-BP020224SVG
CSB-MP020224SVG
CSB-EP020224SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fuscata fuscata Glycophorin-A (GYPA), partial CSB-YP010074MMX1
CSB-EP010074MMX1
CSB-BP010074MMX1
CSB-MP010074MMX1
CSB-EP010074MMX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum DNA-directed RNA polymerase II subunit RPB1 (RPII), partial CSB-YP018327PLS
CSB-EP018327PLS
CSB-BP018327PLS
CSB-MP018327PLS
CSB-EP018327PLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida tropicalis Lanosterol 14-alpha demethylase (ERG11), partial CSB-YP006459CZN
CSB-EP006459CZN
CSB-BP006459CZN
CSB-MP006459CZN
CSB-EP006459CZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antrozous pallidus Hemoglobin subunit alpha (HBA) CSB-YP010147ALV
CSB-EP010147ALV
CSB-BP010147ALV
CSB-MP010147ALV
CSB-EP010147ALV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pteropus alecto Hemoglobin subunit alpha (HBA) CSB-YP010147PWS
CSB-EP010147PWS
CSB-BP010147PWS
CSB-MP010147PWS
CSB-EP010147PWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus ATP synthase subunit a (MT-ATP6), partial CSB-YP015070DXU1
CSB-EP015070DXU1
CSB-BP015070DXU1
CSB-MP015070DXU1
CSB-EP015070DXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Turdus merula Hemoglobin subunit beta (HBB) CSB-YP010150TRE
CSB-EP010150TRE
CSB-BP010150TRE
CSB-MP010150TRE
CSB-EP010150TRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradypus tridactylus Hemoglobin subunit beta (HBB) CSB-YP010150BLF
CSB-EP010150BLF
CSB-BP010150BLF
CSB-MP010150BLF
CSB-EP010150BLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leishmania tarentolae Cytochrome c oxidase subunit 3, partial CSB-YP015074LOI
CSB-EP015074LOI
CSB-BP015074LOI
CSB-MP015074LOI
CSB-EP015074LOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria ATP synthase subunit a (ATP6), partial CSB-YP015070LHY1
CSB-EP015070LHY1
CSB-BP015070LHY1
CSB-MP015070LHY1
CSB-EP015070LHY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anas platyrhynchos Acylphosphatase-2 (ACYP2) CSB-YP001266BZD
CSB-EP001266BZD
CSB-BP001266BZD
CSB-MP001266BZD
CSB-EP001266BZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio hamadryas CD44 antigen (CD44), partial CSB-YP004938ERJ1
CSB-EP004938ERJ1
CSB-BP004938ERJ1
CSB-MP004938ERJ1
CSB-EP004938ERJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ubiquitin-60S ribosomal protein L40 (ubqB) CSB-YP025416DKK
CSB-EP025416DKK
CSB-BP025416DKK
CSB-MP025416DKK
CSB-EP025416DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gelsolin (gsn) CSB-YP009965XBE
CSB-EP009965XBE
CSB-BP009965XBE
CSB-MP009965XBE
CSB-EP009965XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Aspartate aminotransferase (aspC) CSB-YP009681FPM
CSB-EP009681FPM
CSB-BP009681FPM
CSB-MP009681FPM
CSB-EP009681FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide deformylase (def) CSB-YP017707SMV
CSB-EP017707SMV
CSB-BP017707SMV
CSB-MP017707SMV
CSB-EP017707SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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