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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant ATP synthase subunit a (atpB), partial CSB-YP015070SMV1
CSB-EP015070SMV1
CSB-BP015070SMV1
CSB-MP015070SMV1
CSB-EP015070SMV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 ATP synthase subunit alpha (atpA), partial CSB-YP002344SMW
CSB-EP002344SMW
CSB-BP002344SMW
CSB-MP002344SMW
CSB-EP002344SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fus), partial CSB-YP009373SMV
CSB-EP009373SMV
CSB-BP009373SMV
CSB-MP009373SMV
CSB-EP009373SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Ts (tsf) CSB-YP025124SMV
CSB-EP025124SMV
CSB-BP025124SMV
CSB-MP025124SMV
CSB-EP025124SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-6-phosphate isomerase (pgi) CSB-YP009717SMV
CSB-EP009717SMV
CSB-BP009717SMV
CSB-MP009717SMV
CSB-EP009717SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Translation initiation factor IF-2 (infB), partial CSB-YP015164SMW
CSB-EP015164SMW
CSB-BP015164SMW
CSB-MP015164SMW
CSB-EP015164SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose-phosphate pyrophosphokinase 1 (prs1) CSB-YP018776SMV
CSB-EP018776SMV
CSB-BP018776SMV
CSB-MP018776SMV
CSB-EP018776SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose-phosphate pyrophosphokinase 2 (prs2) CSB-YP018779SMV
CSB-EP018779SMV
CSB-BP018779SMV
CSB-MP018779SMV
CSB-EP018779SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP015197SMV
CSB-EP015197SMV
CSB-BP015197SMV
CSB-MP015197SMV
CSB-EP015197SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease 3 (rnc) CSB-YP019809SMV
CSB-EP019809SMV
CSB-BP019809SMV
CSB-MP019809SMV
CSB-EP019809SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-recycling factor (frr) CSB-YP014941SMV
CSB-EP014941SMV
CSB-BP014941SMV
CSB-MP014941SMV
CSB-EP014941SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Serine hydroxymethyltransferase (glyA) CSB-YP021274SMW
CSB-EP021274SMW
CSB-BP021274SMW
CSB-MP021274SMW
CSB-EP021274SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Triosephosphate isomerase (tpiA) CSB-YP024102SMW
CSB-EP024102SMW
CSB-BP024102SMW
CSB-MP024102SMW
CSB-EP024102SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops alternatus L-amino-acid oxidase, partial CSB-YP011660BUS
CSB-EP011660BUS
CSB-BP011660BUS
CSB-MP011660BUS
CSB-EP011660BUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops jararaca L-amino-acid oxidase CSB-YP011660BUW
CSB-EP011660BUW
CSB-BP011660BUW
CSB-MP011660BUW
CSB-EP011660BUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetrahymena thermophila 60S ribosomal protein L13a (RPL13A) CSB-YP020130TKA
CSB-EP020130TKA
CSB-BP020130TKA
CSB-MP020130TKA
CSB-EP020130TKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetrahymena thermophila 60S ribosomal protein L35a (RPL35A) CSB-YP020248TKA
CSB-EP020248TKA
CSB-BP020248TKA
CSB-MP020248TKA
CSB-EP020248TKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetrahymena thermophila 60S ribosomal protein L13 (RPL13) CSB-YP020129TKA
CSB-EP020129TKA
CSB-BP020129TKA
CSB-MP020129TKA
CSB-EP020129TKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eudyptes chrysocome Hemoglobin subunit alpha (HBA) CSB-YP010147EPB
CSB-EP010147EPB
CSB-BP010147EPB
CSB-MP010147EPB
CSB-EP010147EPB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyprinus carpio Beta-crystallin S (crygs) CSB-YP006025EQE
CSB-EP006025EQE
CSB-BP006025EQE
CSB-MP006025EQE
CSB-EP006025EQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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