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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Gorilla gorilla gorilla Ig alpha-1 chain C region (IGHA1) CSB-YP011111GGZ
CSB-EP011111GGZ
CSB-BP011111GGZ
CSB-MP011111GGZ
CSB-EP011111GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Securin (cut2) CSB-YP019074SXV
CSB-EP019074SXV
CSB-BP019074SXV
CSB-MP019074SXV
CSB-EP019074SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin beta chain CSB-YP025318LDQ
CSB-EP025318LDQ
CSB-BP025318LDQ
CSB-MP025318LDQ
CSB-EP025318LDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine synthetase (glnA) CSB-YP009553MSD
CSB-EP009553MSD
CSB-BP009553MSD
CSB-MP009553MSD
CSB-EP009553MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sodium/potassium-transporting ATPase subunit beta-3 (atp1b3), partial CSB-YP002328XBE1
CSB-EP002328XBE1
CSB-BP002328XBE1
CSB-MP002328XBE1
CSB-EP002328XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vulpes vulpes Hemoglobin subunit alpha (HBA) CSB-YP010147VFW
CSB-EP010147VFW
CSB-BP010147VFW
CSB-MP010147VFW
CSB-EP010147VFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Thymidine kinase (21), partial CSB-YP023577SAX
CSB-EP023577SAX
CSB-BP023577SAX
CSB-MP023577SAX
CSB-EP023577SAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rangifer tarandus Hemoglobin subunit beta (HBB) CSB-YP010150RBL
CSB-EP010150RBL
CSB-BP010150RBL
CSB-MP010150RBL
CSB-EP010150RBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Multidrug resistance protein 1 (ABCB1), partial CSB-YP001046DXU
CSB-EP001046DXU
CSB-BP001046DXU
CSB-MP001046DXU
CSB-EP001046DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Substance-K receptor (TACR2), partial CSB-YP023069HU1
CSB-EP023069HU1
CSB-BP023069HU1
CSB-MP023069HU1
CSB-EP023069HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Cytochrome c oxidase subunit 2 (cox2), partial CSB-YP015073SXV1
CSB-EP015073SXV1
CSB-BP015073SXV1
CSB-MP015073SXV1
CSB-EP015073SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varecia variegata Hemoglobin subunit beta (HBB) CSB-YP010150VAN
CSB-EP010150VAN
CSB-BP010150VAN
CSB-MP010150VAN
CSB-EP010150VAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Deoxyribonuclease-1 (Dnase1) CSB-YP007049RA
CSB-EP007049RA
CSB-BP007049RA
CSB-MP007049RA
CSB-EP007049RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akodon boliviensis Cytochrome b (MT-CYB), partial CSB-YP015075AFS
CSB-EP015075AFS
CSB-BP015075AFS
CSB-MP015075AFS
CSB-EP015075AFS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akodon kofordi Cytochrome b (MT-CYB), partial CSB-YP015075AFW
CSB-EP015075AFW
CSB-BP015075AFW
CSB-MP015075AFW
CSB-EP015075AFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akodon subfuscus Cytochrome b (MT-CYB), partial CSB-YP015075AGD
CSB-EP015075AGD
CSB-BP015075AGD
CSB-MP015075AGD
CSB-EP015075AGD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akodon mimus Cytochrome b (MT-CYB), partial CSB-YP015075AFX
CSB-EP015075AFX
CSB-BP015075AFX
CSB-MP015075AFX
CSB-EP015075AFX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cerastes cerastes Phospholipase A2 CSB-YP018091CGI
CSB-EP018091CGI
CSB-BP018091CGI
CSB-MP018091CGI
CSB-EP018091CGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Platichthys flesus Urotensin-2 CSB-YP025771EWX
CSB-EP025771EWX
CSB-BP025771EWX
CSB-MP025771EWX
CSB-EP025771EWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Pyruvate dehydrogenase E1 component subunit alpha (pdhA) CSB-YP017716GFM
CSB-EP017716GFM
CSB-BP017716GFM
CSB-MP017716GFM
CSB-EP017716GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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