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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodobacter capsulatus Adenosylhomocysteinase (ahcY) CSB-YP001474RLD
CSB-EP001474RLD
CSB-BP001474RLD
CSB-MP001474RLD
CSB-EP001474RLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 5-oxoprolinase (OXP1), partial CSB-YP016350SVG
CSB-EP016350SVG
CSB-BP016350SVG
CSB-MP016350SVG
CSB-EP016350SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 5-hydroxytryptamine receptor 2B (5-HT1B), partial CSB-YP010888DLU1
CSB-BP010888DLU1
CSB-MP010888DLU1
CSB-EP010888DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callithrix jacchus Interferon gamma (IFNG) CSB-YP011050CYL
CSB-EP011050CYL
CSB-BP011050CYL
CSB-MP011050CYL
CSB-EP011050CYL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat 5-hydroxytryptamine receptor 1B (Htr1b), partial CSB-YP010882RA1
CSB-EP010882RA1
CSB-BP010882RA1
CSB-MP010882RA1
CSB-EP010882RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Signal peptidase complex subunit 3 (SPC22), partial CSB-YP022517CH1
CSB-EP022517CH1
CSB-BP022517CH1
CSB-MP022517CH1
CSB-EP022517CH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratitis capitata Superoxide dismutase [Cu-Zn] (Sod) CSB-YP022397DRF
CSB-EP022397DRF
CSB-BP022397DRF
CSB-MP022397DRF
CSB-EP022397DRF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana T-complex protein 1 subunit alpha (CCT1), partial CSB-YP023320DOA
CSB-EP023320DOA
CSB-BP023320DOA
CSB-MP023320DOA
CSB-EP023320DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Thymidine kinase (TK) CSB-YP023577IAD
CSB-EP023577IAD
CSB-BP023577IAD
CSB-MP023577IAD
CSB-EP023577IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Ferritin heavy chain (FTH1) CSB-YP009030DXU
CSB-EP009030DXU
CSB-BP009030DXU
CSB-MP009030DXU
CSB-EP009030DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Interleukin-10 (Il10) CSB-YP011580RA
CSB-EP011580RA
CSB-BP011580RA
CSB-MP011580RA
CSB-EP011580RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Triosephosphate isomerase (Tpi) CSB-YP024102DLU
CSB-EP024102DLU
CSB-BP024102DLU
CSB-MP024102DLU
CSB-EP024102DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epimachus albertisi Cytochrome b (MT-CYB), partial CSB-YP015075EET
CSB-EP015075EET
CSB-BP015075EET
CSB-MP015075EET
CSB-EP015075EET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amia calva Cytochrome c oxidase subunit 1 (mt-co1), partial CSB-YP015072AIW1
CSB-EP015072AIW1
CSB-BP015072AIW1
CSB-MP015072AIW1
CSB-EP015072AIW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pantodon buchholzi Cytochrome c oxidase subunit 1 (mt-co1), partial CSB-YP015072PBJ1
CSB-EP015072PBJ1
CSB-BP015072PBJ1
CSB-MP015072PBJ1
CSB-EP015072PBJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gomphosus varius Cytochrome c oxidase subunit 2 (mt-co2), partial CSB-YP015073GCU1
CSB-EP015073GCU1
CSB-BP015073GCU1
CSB-MP015073GCU1
CSB-EP015073GCU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amia calva Cytochrome b (mt-cyb), partial CSB-YP015075AIW
CSB-EP015075AIW
CSB-BP015075AIW
CSB-MP015075AIW
CSB-EP015075AIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scaphirhynchus platorynchus Cytochrome b (mt-cyb), partial CSB-YP015075SDE1
CSB-EP015075SDE1
CSB-BP015075SDE1
CSB-MP015075SDE1
CSB-EP015075SDE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 2 (eef-2), partial CSB-YP007434CXY
CSB-EP007434CXY
CSB-BP007434CXY
CSB-MP007434CXY
CSB-EP007434CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Cytochrome b (MT-CYB), partial CSB-YP015075FIG
CSB-EP015075FIG
CSB-BP015075FIG
CSB-MP015075FIG
CSB-EP015075FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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