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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis F-actin-capping protein subunit alpha-1 (capza1) CSB-YP004510XBE
CSB-EP004510XBE
CSB-BP004510XBE
CSB-MP004510XBE
CSB-EP004510XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hylobates syndactylus Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073HXY1
CSB-EP015073HXY1
CSB-BP015073HXY1
CSB-MP015073HXY1
CSB-EP015073HXY1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio hamadryas Uricase (UOX) CSB-YP025648ERJ
CSB-EP025648ERJ
CSB-BP025648ERJ
CSB-MP025648ERJ
CSB-EP025648ERJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit a (atpB), partial CSB-YP015070BAE1
CSB-EP015070BAE1
CSB-BP015070BAE1
CSB-MP015070BAE1
CSB-EP015070BAE1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serinus canaria N-myc proto-oncogene protein (MYCN) CSB-YP015278SYL
CSB-EP015278SYL
CSB-BP015278SYL
CSB-MP015278SYL
CSB-EP015278SYL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tuf) CSB-YP025342FBZ
CSB-EP025342FBZ
CSB-BP025342FBZ
CSB-MP025342FBZ
CSB-EP025342FBZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba castellanii TATA-box-binding protein (TFIID) CSB-YP023239AAJ
CSB-EP023239AAJ
CSB-BP023239AAJ
CSB-MP023239AAJ
CSB-EP023239AAJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA mismatch repair protein msh3 (msh3), partial CSB-YP015029SXV
CSB-EP015029SXV
CSB-BP015029SXV
CSB-MP015029SXV
CSB-EP015029SXV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073GGZ1
CSB-EP015073GGZ1
CSB-BP015073GGZ1
CSB-MP015073GGZ1
CSB-EP015073GGZ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone H1.1 (At1g06760) CSB-YP010376DOA
CSB-EP010376DOA
CSB-BP010376DOA
CSB-MP010376DOA
CSB-EP010376DOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit alpha (atpA), partial CSB-YP002344ELY
CSB-EP002344ELY
CSB-BP002344ELY
CSB-MP002344ELY
CSB-EP002344ELY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S acidic ribosomal protein P0 CSB-YP020336TQY
CSB-EP020336TQY
CSB-BP020336TQY
CSB-MP020336TQY
CSB-EP020336TQY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha NADH-ubiquinone oxidoreductase chain 1 (ND1), partial CSB-YP015076MCB
CSB-EP015076MCB
CSB-BP015076MCB
CSB-MP015076MCB
CSB-EP015076MCB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. ATP synthase subunit delta (atpH) CSB-YP002355SSQ
CSB-EP002355SSQ
CSB-BP002355SSQ
CSB-MP002355SSQ
CSB-EP002355SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xiphophorus helleri Tyrosine-protein kinase Yes (yes), partial CSB-YP026253XBJ
CSB-EP026253XBJ
CSB-BP026253XBJ
CSB-MP026253XBJ
CSB-EP026253XBJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Phosphatidylserine decarboxylase proenzyme (PISD) CSB-YP018033DXU
CSB-EP018033DXU
CSB-BP018033DXU
CSB-MP018033DXU
CSB-EP018033DXU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Evasterias troschelii Protein Wnt-6 (WNT-6) CSB-YP026140EIS
CSB-EP026140EIS
CSB-BP026140EIS
CSB-MP026140EIS
CSB-EP026140EIS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anser caerulescens Protein Wnt-5a (WNT5A) CSB-YP026138AKY
CSB-EP026138AKY
CSB-BP026138AKY
CSB-MP026138AKY
CSB-EP026138AKY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plestiodon skiltonianus Protein Wnt-5b (WNT-5B) CSB-YP026139PNB
CSB-EP026139PNB
CSB-BP026139PNB
CSB-MP026139PNB
CSB-EP026139PNB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pituophis melanoleucus Protein Wnt-3a (WNT-3A) CSB-YP026136PKZ
CSB-EP026136PKZ
CSB-BP026136PKZ
CSB-MP026136PKZ
CSB-EP026136PKZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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