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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Alpha-soluble NSF attachment protein (SEC17) CSB-YP015447SVG
CSB-EP015447SVG
CSB-BP015447SVG
CSB-MP015447SVG
CSB-EP015447SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase III subunit RPC6 (RPC34) CSB-YP018348SVG
CSB-EP018348SVG
CSB-BP018348SVG
CSB-MP018348SVG
CSB-EP018348SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-glucose 4-epimerase (galE) CSB-YP009197CQF
CSB-EP009197CQF
CSB-BP009197CQF
CSB-MP009197CQF
CSB-EP009197CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Elongation factor Tu (tuf) CSB-YP025342BDQ
CSB-EP025342BDQ
CSB-BP025342BDQ
CSB-MP025342BDQ
CSB-EP025342BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Elongation factor Tu (tuf) CSB-YP025342SGB
CSB-EP025342SGB
CSB-BP025342SGB
CSB-MP025342SGB
CSB-EP025342SGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum ATP synthase subunit delta (atpH) CSB-YP002355MWI
CSB-EP002355MWI
CSB-BP002355MWI
CSB-MP002355MWI
CSB-EP002355MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis 40S ribosomal protein S28 (RPS28) CSB-YP020430KBK
CSB-EP020430KBK
CSB-BP020430KBK
CSB-MP020430KBK
CSB-EP020430KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 26S protease regulatory subunit 6A (RPT5) CSB-YP018891SVG
CSB-EP018891SVG
CSB-BP018891SVG
CSB-MP018891SVG
CSB-EP018891SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mediator of RNA polymerase II transcription subunit 9 (CSE2) CSB-YP013669SVG
CSB-EP013669SVG
CSB-BP013669SVG
CSB-MP013669SVG
CSB-EP013669SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-3 (infC) CSB-YP015166SYN
CSB-EP015166SYN
CSB-BP015166SYN
CSB-MP015166SYN
CSB-EP015166SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles quadrimaculatus NADH-ubiquinone oxidoreductase chain 1 (ND1), partial CSB-YP015076AKU
CSB-EP015076AKU
CSB-BP015076AKU
CSB-MP015076AKU
CSB-EP015076AKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles quadrimaculatus NADH-ubiquinone oxidoreductase chain 5 (ND5), partial CSB-YP015081AKU
CSB-EP015081AKU
CSB-BP015081AKU
CSB-MP015081AKU
CSB-EP015081AKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Cytochrome c oxidase subunit 1 (ctaD), partial CSB-YP015072RLE
CSB-EP015072RLE
CSB-BP015072RLE
CSB-MP015072RLE
CSB-EP015072RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anemia phyllitidis Tubulin beta-1 chain (TUBB1) CSB-YP025319AKC
CSB-EP025319AKC
CSB-BP025319AKC
CSB-MP025319AKC
CSB-EP025319AKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase II subunit RPB7 (RPB7) CSB-YP018334SVG
CSB-EP018334SVG
CSB-BP018334SVG
CSB-MP018334SVG
CSB-EP018334SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 35 (VPS35), partial CSB-YP025904SVG
CSB-EP025904SVG
CSB-BP025904SVG
CSB-MP025904SVG
CSB-EP025904SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formosania lacustre NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078FCK1
CSB-EP015078FCK1
CSB-BP015078FCK1
CSB-MP015078FCK1
CSB-EP015078FCK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formosania lacustre NADH-ubiquinone oxidoreductase chain 6 (MT-ND6), partial CSB-YP015082FCK1
CSB-EP015082FCK1
CSB-BP015082FCK1
CSB-MP015082FCK1
CSB-EP015082FCK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate receptor 1 (glr-1), partial CSB-YP009898CXY
CSB-EP009898CXY
CSB-BP009898CXY
CSB-MP009898CXY
CSB-EP009898CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase kappa (polk-1), partial CSB-YP018317CXY
CSB-EP018317CXY
CSB-BP018317CXY
CSB-MP018317CXY
CSB-EP018317CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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