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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (Aimp1) CSB-YP020871MO
CSB-BP020871MO
CSB-MP020871MO
CSB-EP020871MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Notophthalmus viridescens Homeobox protein Hox-A2 CSB-YP010652NEO
CSB-EP010652NEO
CSB-BP010652NEO
CSB-MP010652NEO
CSB-EP010652NEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein Wnt-7a (wnt7a) CSB-YP026141XBE
CSB-EP026141XBE
CSB-BP026141XBE
CSB-MP026141XBE
CSB-EP026141XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta V-type proton ATPase subunit B (VHA55) CSB-YP002398MPV
CSB-EP002398MPV
CSB-BP002398MPV
CSB-MP002398MPV
CSB-EP002398MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster V-type proton ATPase subunit B (Vha55) CSB-YP002398DLU
CSB-EP002398DLU
CSB-BP002398DLU
CSB-MP002398DLU
CSB-EP002398DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta V-type proton ATPase subunit F (VHA14) CSB-YP002407MPV
CSB-EP002407MPV
CSB-BP002407MPV
CSB-MP002407MPV
CSB-EP002407MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203DZQ
CSB-EP001203DZQ
CSB-BP001203DZQ
CSB-MP001203DZQ
CSB-EP001203DZQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Poly [ADP-ribose] polymerase 1 (parp1), partial CSB-YP017457XBE
CSB-EP017457XBE
CSB-BP017457XBE
CSB-MP017457XBE
CSB-EP017457XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli GDP-L-fucose synthase (fcl) CSB-YP025200ENV
CSB-EP025200ENV
CSB-BP025200ENV
CSB-MP025200ENV
CSB-EP025200ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Coatomer subunit gamma (SEC21), partial CSB-YP005786SVG
CSB-EP005786SVG
CSB-BP005786SVG
CSB-MP005786SVG
CSB-EP005786SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant TATA-box-binding protein (tbp-1) CSB-YP023239CXY
CSB-EP023239CXY
CSB-BP023239CXY
CSB-MP023239CXY
CSB-EP023239CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mediator of RNA polymerase II transcription subunit 16 (SIN4), partial CSB-YP013649SVG
CSB-EP013649SVG
CSB-BP013649SVG
CSB-MP013649SVG
CSB-EP013649SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Trehalase, partial CSB-YP024402BTT
CSB-EP024402BTT
CSB-BP024402BTT
CSB-MP024402BTT
CSB-EP024402BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Actin-related protein 2 (ARP2) CSB-YP001248SVG
CSB-EP001248SVG
CSB-BP001248SVG
CSB-MP001248SVG
CSB-EP001248SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae H/ACA ribonucleoprotein complex subunit 2 (NHP2) CSB-YP015793SVG
CSB-EP015793SVG
CSB-BP015793SVG
CSB-MP015793SVG
CSB-EP015793SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Translation initiation factor eIF-2B subunit epsilon (GCD6), partial CSB-YP007518SVG
CSB-EP007518SVG
CSB-BP007518SVG
CSB-MP007518SVG
CSB-EP007518SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mediator of RNA polymerase II transcription subunit 17 (SRB4), partial CSB-YP013650SVG
CSB-EP013650SVG
CSB-BP013650SVG
CSB-MP013650SVG
CSB-EP013650SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae U3 small nucleolar ribonucleoprotein protein IMP3 (IMP3) CSB-YP011692SVG
CSB-EP011692SVG
CSB-BP011692SVG
CSB-MP011692SVG
CSB-EP011692SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variola virus DNA topoisomerase 1 (TOP1) CSB-YP024056VAR
CSB-EP024056VAR
CSB-BP024056VAR
CSB-MP024056VAR
CSB-EP024056VAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Elongation factor Tu (tuf) CSB-YP025342FPY
CSB-EP025342FPY
CSB-BP025342FPY
CSB-MP025342FPY
CSB-EP025342FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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