Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Zinc transporter ZIP1 (slc39a1), partial CSB-YP021625DIL
CSB-EP021625DIL
CSB-BP021625DIL
CSB-MP021625DIL
CSB-EP021625DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Single-stranded DNA-binding protein (ssb) CSB-YP022706HCO
CSB-EP022706HCO
CSB-BP022706HCO
CSB-MP022706HCO
CSB-EP022706HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Neutrophil defensin 3 CSB-YP006655MOW
CSB-EP006655MOW
CSB-BP006655MOW
CSB-MP006655MOW
CSB-EP006655MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Ubiquitin carboxyl-terminal hydrolase 14 (USP14) CSB-YP025704EQV
CSB-EP025704EQV
CSB-BP025704EQV
CSB-MP025704EQV
CSB-EP025704EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA pseudouridine synthase A (truA) CSB-YP019086RLU
CSB-EP019086RLU
CSB-BP019086RLU
CSB-MP019086RLU
CSB-EP019086RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582RLU
CSB-EP009582RLU
CSB-BP009582RLU
CSB-MP009582RLU
CSB-EP009582RLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Guanylate kinase (gmk) CSB-YP010059BFP
CSB-EP010059BFP
CSB-BP010059BFP
CSB-MP010059BFP
CSB-EP010059BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Guanylate kinase (gmk) CSB-YP010059OBN
CSB-EP010059OBN
CSB-BP010059OBN
CSB-MP010059OBN
CSB-EP010059OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hylobates lar Interleukin-2 (IL2) CSB-YP011629HXW
CSB-EP011629HXW
CSB-BP011629HXW
CSB-MP011629HXW
CSB-EP011629HXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Cystatin-B (CSTB) CSB-YP006100EQU
CSB-EP006100EQU
CSB-BP006100EQU
CSB-MP006100EQU
CSB-EP006100EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agmatinase (speB) CSB-YP001447SZB
CSB-EP001447SZB
CSB-BP001447SZB
CSB-MP001447SZB
CSB-EP001447SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator Single-stranded DNA-binding protein (ssb) CSB-YP022706DZQ
CSB-EP022706DZQ
CSB-BP022706DZQ
CSB-MP022706DZQ
CSB-EP022706DZQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Single-stranded DNA-binding protein (ssb) CSB-YP022706RDR
CSB-EP022706RDR
CSB-BP022706RDR
CSB-MP022706RDR
CSB-EP022706RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Histamine H2 receptor (HRH2), partial CSB-YP010738EQV1
CSB-EP010738EQV1
CSB-BP010738EQV1
CSB-MP010738EQV1
CSB-EP010738EQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes General transcription factor IIH subunit 4 (GTF2H4) CSB-YP010013EQV
CSB-EP010013EQV
CSB-BP010013EQV
CSB-MP010013EQV
CSB-EP010013EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens tRNA pseudouridine synthase A (truA) CSB-YP019086GBK
CSB-EP019086GBK
CSB-BP019086GBK
CSB-MP019086GBK
CSB-EP019086GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NADH-ubiquinone oxidoreductase chain 6 (ND6), partial CSB-YP015082DOA1
CSB-EP015082DOA1
CSB-BP015082DOA1
CSB-MP015082DOA1
CSB-EP015082DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Guanylate kinase (gmk) CSB-YP010059LAW
CSB-EP010059LAW
CSB-BP010059LAW
CSB-MP010059LAW
CSB-EP010059LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Agmatinase (speB) CSB-YP001447NGF
CSB-EP001447NGF
CSB-BP001447NGF
CSB-MP001447NGF
CSB-EP001447NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipoyl synthase (lipA) CSB-YP012927ENV
CSB-EP012927ENV
CSB-BP012927ENV
CSB-MP012927ENV
CSB-EP012927ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>