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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Colobus guereza Lysozyme C (LYZ) CSB-YP013283DVH
CSB-EP013283DVH
CSB-BP013283DVH
CSB-MP013283DVH
CSB-EP013283DVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844PYX1
CSB-EP004844PYX1
CSB-BP004844PYX1
CSB-MP004844PYX1
CSB-EP004844PYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Hemoglobin subunit delta (HBD) CSB-YP010152EXP
CSB-EP010152EXP
CSB-BP010152EXP
CSB-MP010152EXP
CSB-EP010152EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Transforming growth factor beta-2 (TGFB2) CSB-YP023448DSU
CSB-EP023448DSU
CSB-BP023448DSU
CSB-MP023448DSU
CSB-EP023448DSU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bison bison Ribonuclease pancreatic (RNASE1) CSB-YP019789BTE
CSB-EP019789BTE
CSB-BP019789BTE
CSB-MP019789BTE
CSB-EP019789BTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Superoxide dismutase [Cu-Zn] (Sod) CSB-YP022397DLU
CSB-EP022397DLU
CSB-BP022397DLU
CSB-MP022397DLU
CSB-EP022397DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 2 (fusA), partial CSB-YP007434FHZ
CSB-EP007434FHZ
CSB-BP007434FHZ
CSB-MP007434FHZ
CSB-EP007434FHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malate dehydrogenase (mdh) CSB-YP013623KAJ
CSB-EP013623KAJ
CSB-BP013623KAJ
CSB-MP013623KAJ
CSB-EP013623KAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Hemoglobin subunit gamma-1 (HBG1) CSB-YP010155EQV
CSB-EP010155EQV
CSB-BP010155EQV
CSB-MP010155EQV
CSB-EP010155EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hylobates lar Hemoglobin subunit gamma-1 (HBG1) CSB-YP010155HXW
CSB-EP010155HXW
CSB-BP010155HXW
CSB-MP010155HXW
CSB-EP010155HXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcription initiation factor IIB (tfb) CSB-YP010001FHZ
CSB-EP010001FHZ
CSB-BP010001FHZ
CSB-MP010001FHZ
CSB-EP010001FHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 60S ribosomal protein L41 (RPL41) CSB-YP020290NHE
CSB-EP020290NHE
CSB-BP020290NHE
CSB-MP020290NHE
CSB-EP020290NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare 60S ribosomal protein L41 (RPL41) CSB-YP020290HWQ
CSB-EP020290HWQ
CSB-BP020290HWQ
CSB-MP020290HWQ
CSB-EP020290HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Neuronal calcium sensor 1 (Ncs1) CSB-YP008983RA
CSB-EP008983RA
CSB-BP008983RA
CSB-MP008983RA
CSB-EP008983RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine/threonine-protein kinase PLK1 (plk1), partial CSB-YP018193XBF
CSB-EP018193XBF
CSB-BP018193XBF
CSB-MP018193XBF
CSB-EP018193XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio 40S ribosomal protein S8 (rps8) CSB-YP020478DIL
CSB-EP020478DIL
CSB-BP020478DIL
CSB-MP020478DIL
CSB-EP020478DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia pahangi 40S ribosomal protein S13 (RPS13) CSB-YP020370BWW
CSB-EP020370BWW
CSB-BP020370BWW
CSB-MP020370BWW
CSB-EP020370BWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Notothenia angustata Hemoglobin subunit alpha-2 (hba2) CSB-YP010148NEP
CSB-EP010148NEP
CSB-BP010148NEP
CSB-MP010148NEP
CSB-EP010148NEP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoserine aminotransferase (serC) CSB-YP018838SWW
CSB-EP018838SWW
CSB-BP018838SWW
CSB-MP018838SWW
CSB-EP018838SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Beta-crystallin B2 (CRYBB2) CSB-YP006013MRG
CSB-EP006013MRG
CSB-BP006013MRG
CSB-MP006013MRG
CSB-EP006013MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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