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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Electron transfer flavoprotein subunit beta (etfB) CSB-YP007845MVZ
CSB-EP007845MVZ
CSB-BP007845MVZ
CSB-MP007845MVZ
CSB-EP007845MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SKX
CSB-EP015150SKX
CSB-BP015150SKX
CSB-MP015150SKX
CSB-EP015150SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SMI
CSB-EP015150SMI
CSB-BP015150SMI
CSB-MP015150SMI
CSB-EP015150SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717MVZ
CSB-EP009717MVZ
CSB-BP009717MVZ
CSB-MP009717MVZ
CSB-EP009717MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-6-phosphate isomerase (pgi) CSB-YP009717SMI
CSB-EP009717SMI
CSB-BP009717SMI
CSB-MP009717SMI
CSB-EP009717SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Glycine cleavage system H protein (gcvH) CSB-YP009335TIW
CSB-EP009335TIW
CSB-BP009335TIW
CSB-MP009335TIW
CSB-EP009335TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Glucokinase (glk) CSB-YP009319NGF
CSB-EP009319NGF
CSB-BP009319NGF
CSB-MP009319NGF
CSB-EP009319NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA) CSB-YP009582EOD
CSB-EP009582EOD
CSB-BP009582EOD
CSB-MP009582EOD
CSB-EP009582EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582SMP
CSB-EP009582SMP
CSB-BP009582SMP
CSB-MP009582SMP
CSB-EP009582SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582SMU
CSB-EP009582SMU
CSB-BP009582SMU
CSB-MP009582SMU
CSB-EP009582SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphobilinogen deaminase (hemC) CSB-YP010524MVZ
CSB-EP010524MVZ
CSB-BP010524MVZ
CSB-MP010524MVZ
CSB-EP010524MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphobilinogen deaminase (hemC) CSB-YP010524MVH
CSB-EP010524MVH
CSB-BP010524MVH
CSB-MP010524MVH
CSB-EP010524MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Hypoxia-inducible factor 1-alpha inhibitor (hif1an) CSB-YP010352DIL
CSB-EP010352DIL
CSB-BP010352DIL
CSB-MP010352DIL
CSB-EP010352DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Single-stranded DNA-binding protein (ssb) CSB-YP022706WBQ
CSB-EP022706WBQ
CSB-BP022706WBQ
CSB-MP022706WBQ
CSB-EP022706WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Polyadenylate-binding protein 5 (PABPC5) CSB-YP017359GGZ
CSB-EP017359GGZ
CSB-BP017359GGZ
CSB-MP017359GGZ
CSB-EP017359GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Superoxide dismutase [Cu-Zn] (SOD1) CSB-YP022397EQV
CSB-EP022397EQV
CSB-BP022397EQV
CSB-MP022397EQV
CSB-EP022397EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Triosephosphate isomerase (TPI1) CSB-YP024102EQV
CSB-EP024102EQV
CSB-BP024102EQV
CSB-MP024102EQV
CSB-EP024102EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma tRNA pseudouridine synthase A (truA) CSB-YP019086OBN
CSB-EP019086OBN
CSB-BP019086OBN
CSB-MP019086OBN
CSB-EP019086OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Gamma-aminobutyric acid receptor-associated protein (Gabarap) CSB-YP009130RA
CSB-EP009130RA
CSB-BP009130RA
CSB-MP009130RA
CSB-EP009130RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canis latrans Hemoglobin subunit beta (HBB) CSB-YP010150CCV
CSB-EP010150CCV
CSB-BP010150CCV
CSB-MP010150CCV
CSB-EP010150CCV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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