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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chlorocebus aethiops CD151 antigen (CD151), partial CSB-YP004880DSU1
CSB-EP004880DSU1
CSB-BP004880DSU1
CSB-MP004880DSU1
CSB-EP004880DSU1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoyl synthase (lipA) CSB-YP012927MLA
CSB-EP012927MLA
CSB-BP012927MLA
CSB-MP012927MLA
CSB-EP012927MLA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fuscata fuscata Protamine-2 (PRM2) CSB-YP018729MMX
CSB-EP018729MMX
CSB-BP018729MMX
CSB-MP018729MMX
CSB-EP018729MMX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio anubis Beta-defensin 1 (DEFB1) CSB-YP006662ERI
CSB-EP006662ERI
CSB-BP006662ERI
CSB-MP006662ERI
CSB-EP006662ERI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis 40S ribosomal protein S11 (RPS11) CSB-YP020365MOV
CSB-EP020365MOV
CSB-BP020365MOV
CSB-MP020365MOV
CSB-EP020365MOV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema denticola Ribosome-recycling factor (frr) CSB-YP014941TPP
CSB-EP014941TPP
CSB-BP014941TPP
CSB-MP014941TPP
CSB-EP014941TPP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens Elongation factor Ts (tsf) CSB-YP025124GBK
CSB-EP025124GBK
CSB-BP025124GBK
CSB-MP025124GBK
CSB-EP025124GBK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Elongation factor Ts (tsf) CSB-YP025124RLU
CSB-EP025124RLU
CSB-BP025124RLU
CSB-MP025124RLU
CSB-EP025124RLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio 60S ribosomal protein L36a (rpl36a) CSB-YP020253DIL
CSB-EP020253DIL
CSB-BP020253DIL
CSB-MP020253DIL
CSB-EP020253DIL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Argininosuccinate synthase (argG) CSB-YP002234CQF
CSB-EP002234CQF
CSB-BP002234CQF
CSB-MP002234CQF
CSB-EP002234CQF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Argininosuccinate synthase (argG) CSB-YP002234MSB
CSB-EP002234MSB
CSB-BP002234MSB
CSB-MP002234MSB
CSB-EP002234MSB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Lysozyme C (LYZ) CSB-YP013283EQU
CSB-EP013283EQU
CSB-BP013283EQU
CSB-MP013283EQU
CSB-EP013283EQU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Hemoglobin subunit delta (HBD) CSB-YP010152EQV
CSB-EP010152EQV
CSB-BP010152EQV
CSB-MP010152EQV
CSB-EP010152EQV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080EXP1
CSB-EP015080EXP1
CSB-BP015080EXP1
CSB-MP015080EXP1
CSB-EP015080EXP1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Ribonuclease pancreatic (RNASE1) CSB-YP019789DSU
CSB-EP019789DSU
CSB-BP019789DSU
CSB-MP019789DSU
CSB-EP019789DSU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Superoxide dismutase [Cu-Zn] (Sod) CSB-YP022397DMG
CSB-EP022397DMG
CSB-BP022397DMG
CSB-MP022397DMG
CSB-EP022397DMG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malate dehydrogenase (mdh) CSB-YP013623YAS
CSB-EP013623YAS
CSB-BP013623YAS
CSB-MP013623YAS
CSB-EP013623YAS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malate dehydrogenase (mdh) CSB-YP013623KBE
CSB-EP013623KBE
CSB-BP013623KBE
CSB-MP013623KBE
CSB-EP013623KBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans Flap endonuclease 1 (fen) CSB-YP008585NAQ
CSB-EP008585NAQ
CSB-BP008585NAQ
CSB-MP008585NAQ
CSB-EP008585NAQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malate dehydrogenase (mdh) CSB-YP013623MLA
CSB-EP013623MLA
CSB-BP013623MLA
CSB-MP013623MLA
CSB-EP013623MLA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過(guò)多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn)，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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